Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons

The Plant Journal : for Cell and Molecular Biology
Wei ChiLixin Zhang

Abstract

The pentatricopeptide-repeat (PPR) protein DELAYED GREENING 1 (DG1) has been shown to be involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis. To gain insight into the mode of DG1 action, we used a yeast two-hybrid screening approach and identified a partner, SIG6, which is a chloroplast sigma factor responsible for the transcription of plastid-encoded RNA polymerase (PEP)-dependent chloroplast genes in cotyledons. Further analysis showed that the C-terminal region of DG1 and the N-terminal region of SIG6 are responsible for such interactions. High-level expression of a truncated C-terminal DG1 in wild-type Arabidopsis caused a dominant-negative phenotype. The sig6 dg1 double mutant displayed a more severe chlorotic phenotype, and the PEP-dependent chloroplast gene transcripts were greatly reduced compared with transcript levels in the single mutants. Overexpression of SIG6 rescued the chlorophyll deficiency in dg1 cotyledons but not in young leaves. In addition, increased SIG6 promoted PEP-dependent chloroplast gene transcript accumulation in the dg1 mutant background. These results suggest that the interaction of DG1 and SIG6 is functionally significant in the regulation o...Continue Reading

References

Oct 1, 1993·Plant Physiology·J E Mullet
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y KovtunJ Sheen
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S L DoveA Hochschild
Feb 27, 2003·Plant Molecular Biology·Isabelle PrivatSilva Mache-Lerbs
Jun 14, 2003·Plant Physiology·Junlan YaoLori A Allison
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuichi TsunoyamaTakashi Shiina
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·H. E. Neuhaus, M. J. Emes
Apr 17, 2004·The Plant Journal : for Cell and Molecular Biology·Carola EmanuelThomas Börner
Aug 13, 2005·The International Journal of Developmental Biology·Enrique Lopez-Juez, Kevin A Pyke
Sep 15, 2005·International Review of Cytology·Takashi ShiinaMuhammad S Khan
Oct 26, 2005·Nucleic Acids Research·Jean-Jacques FavorySilva Lerbs-Mache
Feb 9, 2006·Cellular and Molecular Life Sciences : CMLS·T Shikanai
Apr 1, 1987·Plant Physiology·E K Archer, H T Bonnett
Jul 11, 2006·Plant Physiology·Eric RivalsAlain Lecharny
Aug 15, 2006·Plant Physiology·Heike LoschelderGerhard Link
Sep 5, 2006·Journal of Molecular Biology·Vitaly CitovskyTzvi Tzfira
Sep 30, 2006·Plant Molecular Biology·Shai KoussevitzkyJoanne Chory
Dec 19, 2006·Nucleic Acids Research·Wafa ZghidiSilva Lerbs-Mache
Mar 16, 2007·Plant Cell Reports·Eugene A Lysenko
Jul 29, 2008·The Plant Journal : for Cell and Molecular Biology·Anne-Laure Chateigner-BoutinPatricia León
Nov 14, 2008·Trends in Plant Science·Christian Schmitz-Linneweber, Ian Small
May 19, 2009·Plant Physiology·Wenhe CaiLixin Zhang
Sep 12, 2009·The EMBO Journal·Mark T Waters, Jane A Langdale
Jan 22, 2010·The Plant Journal : for Cell and Molecular Biology·Jennifer SchweerGerhard Link

❮ Previous
Next ❯

Citations

Feb 19, 2011·Plant Physiology·Barry J Pogson, Verónica Albrecht
Apr 29, 2014·Plant Science : an International Journal of Experimental Plant Biology·Damiano GabottiGabriella Consonni
Sep 7, 2012·The Plant Journal : for Cell and Molecular Biology·Jesse D WoodsonJoanne Chory
Feb 15, 2015·Biochimica Et Biophysica Acta·Thomas BörnerVictor V Kusnetsov
Jan 18, 2015·Biochimica Et Biophysica Acta·Wei ChiLixin Zhang
Apr 29, 2015·Journal of Hazardous Materials·Yang-Er ChenShu Yuan
Jul 30, 2014·Frontiers in Plant Science·Qing-Bo YuZhong-Nan Yang
Apr 4, 2019·PloS One·Juliana Andrea Pérez Di GiorgioNormand Brisson
Feb 10, 2021·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Sujith PuthiyaveetilIskander M Ibrahim
Jun 9, 2020·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jingliang SunJian-Xiang Liu
Apr 4, 2021·International Journal of Molecular Sciences·Xinwei WangJianwei Xiao
May 27, 2021·Plant Science : an International Journal of Experimental Plant Biology·Xinwei WangJianwei Xiao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.