PMID: 9177184Jun 10, 1997Paper

Interaction of the S phase regulator cdc18 with cyclin-dependent kinase in fission yeast

Proceedings of the National Academy of Sciences of the United States of America
G W BrownT J Kelly

Abstract

The fission yeast gene cdc18(+) is required for entry into S phase and for coupling mitosis to the successful completion of S phase. Cdc18 is a highly unstable protein that is expressed only once per cell cycle at the G1/S boundary. Overexpression of Cdc18 causes a mitotic delay and reinitiation of DNA replication, suggesting that the inactivation of Cdc18 plays a role in preventing rereplication within a given cell cycle. In this paper, we present evidence that Cdc18 is associated with active cyclin-dependent kinase in vivo. We have expressed Cdc18 as a glutathione S-transferase fusion in fission yeast and demonstrated that the fusion protein is functional in vivo. We find that the Cdc18 fusion protein copurifies with a kinase activity capable of phosphorylating histone H1 and Cdc18. The activity was identified by a variety of methods as the cyclin-dependent kinase containing the product of the cdc2(+) gene. The amino terminus of Cdc18 is required for association with cyclin-dependent kinase, but the association does not require the consensus cyclin-dependent kinase phosphorylation sites in this region. Additionally, both G1/S and mitotic forms of cyclin-dependent kinase phosphorylate and interact with Cdc18. These interaction...Continue Reading

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Citations

Jun 1, 1997·Current Opinion in Cell Biology·P V Jallepalli, T J Kelly
Jun 26, 1998·Current Opinion in Cell Biology·P Pasero, S M Gasser
Jan 23, 1999·Current Opinion in Cell Biology·J Leatherwood
Jun 4, 1998·Current Opinion in Genetics & Development·D M Gilbert
Aug 16, 2002·Cell Death and Differentiation·W C BurhansH Baumann
Feb 16, 2002·European Journal of Biochemistry·Esther BiermannRolf Knippers
Aug 6, 1999·Molecular Biology of the Cell·U HerbigE Fanning
Mar 14, 1998·Molecular Biology of the Cell·A Lopez-GironaP Russell
Nov 14, 1997·Genes & Development·P V JallepalliT J Kelly
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Susan L Forsburg
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·P V JallepalliT J Kelly
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·W JiangT Hunter
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·C S Detweiler, J J Li
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M WeinreichB Stillman
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·M WeinreichB Stillman
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J TianW J Lennarz
Feb 13, 2001·Current Opinion in Genetics & Development·K Labib, J F Diffley
Apr 21, 2004·The Journal of Cell Biology·Maren OehlmannJ Julian Blow
Aug 31, 2000·Annual Review of Biochemistry·T J Kelly, G W Brown
Aug 15, 1998·The Journal of Biological Chemistry·G W Brown, T J Kelly
May 10, 2017·Molecular Cancer Research : MCR·Tara H W DobsonVidya Gopalakrishnan

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