Interaction of toxic azo dyes with heme protein: biophysical insights into the binding aspect of the food additive amaranth with human hemoglobin

Journal of Hazardous Materials
Anirban Basu, Gopinatha Suresh Kumar

Abstract

A biophysical study on the interaction of the food colorant amaranth with hemoglobin was undertaken. Spectrophotometric and spectrofluorimetric studies proposed for an intimate binding interaction between the dye and the protein. The dye quenched the fluorescence of the protein remarkably and the mechanism of quenching was found to be static in nature. Synchronous fluorescence studies suggested that the polarity around the tryptophan residues was altered in the presence of amaranth whereas the polarity around tyrosine residues remained largely unaltered. 3D fluorescence, FTIR and circular dichroism results suggested that the binding reaction caused conformational changes in hemoglobin. The negative far-UV CD bands exhibited a significantly large decrease in magnitude in the presence of amaranth. From calorimetry studies it was established that the binding was driven by a large positive entropic contribution and a small but favorable enthalpy change.

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Apr 20, 2014·Journal of Hazardous Materials·Anirban Basu, Gopinatha Suresh Kumar
Jun 26, 2014·Food & Function·Anirban Basu, Gopinatha Suresh Kumar

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Citations

Apr 12, 2016·Journal of Biomolecular Structure & Dynamics·Debipreeta Bhowmik, Gopinatha Suresh Kumar
Apr 20, 2016·Journal of Biomolecular Structure & Dynamics·Paromita SealRajen Haldar
Oct 22, 2016·Journal of Photochemistry and Photobiology. B, Biology·Anirban Basu, Gopinatha Suresh Kumar
Jan 18, 2017·The Journal of Physical Chemistry. B·Anirban Basu, Gopinatha Suresh Kumar
Mar 7, 2017·Food & Function·Natália MartinsIsabel C F R Ferreira
Nov 6, 2018·Environmental Science and Pollution Research International·Luyi ZouYue Teng
Jan 21, 2020·International Journal of Biological Macromolecules·Zeynep Mine ŞenolNihat Karakuş
Nov 28, 2017·International Journal of Biological Macromolecules·Fatemeh KazemiAli Akbar Saboury

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