Interaction of transcription factors with serum response factor. Identification of the Elk-1 binding surface.

The Journal of Biological Chemistry
Y LingA D Sharrocks

Abstract

Serum response elements (SREs) play important roles in transforming extracellular signals into specific nuclear responses. The SRE-binding protein, serum response factor (SRF), plays a pivotal role in this process. Several transcription factors have been shown to interact with SRF and thereby create distinct complexes with different regulatory potentials. The ETS domain transcription factor Elk-1 is one such protein and serves to integrate distinct mitogen-activated protein kinase cascades at SREs. Elk-1 uses a short hydrophobic surface presented on the surface of an alpha-helix to interact with SRF. In this study we have used site-directed mutagenesis to identify residues in SRF that comprise the Elk-1 binding surface. The Elk-1 binding surface is composed of residues that lie on a hydrophobic surface-exposed groove located at the junction of the MADS box and C-terminal SAM motif. Different residues are implicated in interactions between SRF and the transcription factor Fli-1, indicating that although some overlap with the Elk-1 binding surface occurs, their interaction surfaces on SRF are distinct. Our data are consistent with the hypothesis that the SRF DNA-binding domain acts as docking site for multiple transcription facto...Continue Reading

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Citations

Dec 25, 2003·Gene·Gilles BuchwalterBohdan Wasylyk
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Sep 27, 2003·Journal of Neuroimmunology·Tudor D BadeaMoon L Shin
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Jul 23, 1999·Current Opinion in Oncology·H KovarA Zoubek
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Dinakar IyerRobert J Schwartz
Mar 3, 2011·Biochimica Et Biophysica Acta·Ozlem DemirIsil Aksan Kurnaz
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Jul 30, 2019·Wellcome Open Research·Ian Prise, Andrew D Sharrocks
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May 2, 2002·The Journal of Biological Chemistry·Jorge L SepulvedaRobert J Schwartz
Jun 18, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick CostelloRichard Treisman

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