Abstract
The tryptic cleavage pattern of transducin (Gt) in solution was compared with that in the presence of phospholipid vesicles, rod outer segment (ROS) membranes kept in the dark, or ROS membranes containing light-activated rhodopsin, metarhodopsin II (Rh*). When Gt was in the high affinity complex with Rh*, the alphat subunit was almost completely protected from proteolysis. The protection of alphat at Arg310 was complete, while Arg204 was substantially protected. The cleavage of alphat at Lys18 was protected in the presence of phospholipid vesicles, ROS membranes kept in the dark, or ROS membranes containing Rh*. The cleavage of betat was slower in the presence of ROS membranes or phospholipid vesicles. When the Rh*. Gt complex was incubated with guanyl-5'-yl thiophosphate, a guanine nucleotide analog known to release the high affinity interaction between Gt and Rh*, the protection at Arg310 and Arg204 was diminished. From our results, we propose that Rh* either physically blocks access of trypsin to Arg204 and Arg310 or maintains the heterotrimer in such a conformation that these cleavage sites are not available. Since Arg204 is involved in the switch interface with betagammat (Lambright, D. G., Sondek, J., Bohm, A., Skiba, N. ...Continue Reading
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 7, 1976·Analytical Biochemistry·M M Bradford
Oct 6, 1992·Biochemistry·J A Malinski, T G Wensel
Oct 22, 1992·Nature·K KokameY Shimonishi
Dec 12, 1989·Biochemistry·M R Mazzoni, H E Hamm
Oct 1, 1989·European Journal of Biochemistry·F BornancinM Chabre
Dec 31, 1985·Biochemistry·G YamanakaL Stryer
Aug 12, 1988·Science·H E HammK P Hofmann
Aug 15, 1970·Nature·U K Laemmli
Aug 1, 1984·The Journal of General Physiology·H E Hamm, M D Bownds
Feb 7, 1980·Nature·H Kühn
Jun 21, 1982·FEBS Letters·D EmeisK P Hofmann
Nov 15, 1983·European Journal of Biochemistry·C PfisterM Chabre
Jan 1, 1983·Methods in Enzymology·L StryerB K Fung
Aug 1, 1984·The Journal of General Physiology·P L WittM D Bownds
Nov 16, 1984·Science·J B HurleyA G Gilman
Apr 1, 1995·Neuropharmacology·L Hein, B K Kobilka
Aug 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Rens-Domiano, H E Hamm
Jan 13, 1995·The Journal of Biological Chemistry·P B WedegaertnerH R Bourne
Jul 1, 1994·Biophysical Journal·J KimS McLaughlin
Nov 29, 1994·Biochemistry·J BigayM Chabre
Nov 17, 1994·Nature·J SondekP B Sigler
Sep 2, 1994·Science·D E ColemanS R Sprang
May 13, 1994·Journal of Molecular Biology·D E ColemanS R Sprang
Jun 23, 1994·Nature·D G LambrightP B Sigler
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T C ThomasE J Neer
Dec 16, 1993·Nature·J P NoelP B Sigler
May 20, 1993·Nature·B R ConklinH R Bourne
Jan 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P A HargraveK P Hofmann
Apr 1, 1993·Journal of Protein Chemistry·M R Mazzoni, H E Hamm
Jan 1, 1993·Annual Review of Pharmacology and Toxicology·H K Yamane, B K Fung
Dec 15, 1995·Cell·M A WallS R Sprang
Jan 5, 1996·The Journal of Biological Chemistry·N P SkibaH E Hamm
Jan 25, 1996·Nature·D G LambrightP B Sigler
Citations
Dec 3, 1999·Proteins·F FanelliP G De Benedetti
Nov 8, 2001·Archives of Biochemistry and Biophysics·J BubisC Möller
Feb 2, 2010·Journal of Molecular Modeling·Andrea Strasser, Hans-Joachim Wittmann
Jan 15, 1999·Pharmacology & Therapeutics·J Wess
Dec 15, 2011·Chemical Reviews·Francesca Fanelli, Pier G De Benedetti
Nov 29, 2007·Nature Reviews. Molecular Cell Biology·William M Oldham, Heidi E Hamm
Jun 15, 2000·Journal of Neurochemistry·T KasaharaY Fukada
Jan 5, 2013·The Journal of Biological Chemistry·Wen MaoJeannie Chen
May 12, 2000·Critical Care Medicine·R A Forse
Mar 3, 2012·PLoS Pathogens·Dustin E BoschDavid P Siderovski
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·K CaiH G Khorana
Dec 3, 2013·Nature Structural & Molecular Biology·Nathan S AlexanderJens Meiler
Oct 27, 2007·Protein Science : a Publication of the Protein Society·Andrew F Neuwald
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Ana KosoyJosé Bubis
Feb 27, 2010·Journal of Molecular Biology·Nikolaos G Sgourakis, Angel E Garcia
Sep 11, 2013·Biochimica Et Biophysica Acta·Irina S Moreira
Jan 18, 2003·Brazilian Journal of Biology = Revista Brasleira De Biologia·R MazzoniN Fenerich-Verani
Aug 8, 2006·Nature Structural & Molecular Biology·William M OldhamHeidi E Hamm
Jul 17, 1998·Nature·T IiriH R Bourne
Jul 22, 2004·Molecular Pharmacology·Arne HeydornEvi Kostenis
Jan 24, 1998·The Journal of Biological Chemistry·H BaeS G Graber
Oct 6, 2000·The Journal of Biological Chemistry·L ArisH E Hamm
Apr 6, 2001·The Journal of Biological Chemistry·E P MarinT P Sakmar
Oct 12, 2000·The Journal of Biological Chemistry·J BlahosJ P Pin
Jun 17, 1998·The Journal of Biological Chemistry·A GilchristH E Hamm
Feb 14, 1998·The Journal of Biological Chemistry·H E Hamm
May 18, 1999·The Journal of Biological Chemistry·H BaeH E Hamm
Jun 28, 2003·The Journal of Biological Chemistry·Michaela HavlickovaJean-Philippe Pin
Mar 13, 1999·The Journal of Biological Chemistry·M NatochinN O Artemyev
Mar 7, 2014·The Journal of Biological Chemistry·Tarjani M ThakerT M Iverson
May 18, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Stefania AlbrizioAnna Maria D'Ursi
Sep 26, 2002·FEBS Letters·Pere Garriga, Joan Manyosa
Apr 13, 1999·The American Journal of Physiology·K KitamuraR T Miller
Apr 8, 1997·Biochemistry·N O Artemyev
Sep 15, 2005·Chemical Reviews·Francesca Fanelli, Pier G De Benedetti
Oct 25, 2006·Biochemistry·Najmoutin G AbdulaevKevin D Ridge