PMID: 9536439Apr 16, 1998Paper

Interaction of troponin-H and glutathione S-transferase-2 in the indirect flight muscles of Drosophila melanogaster

Journal of Muscle Research and Cell Motility
J D ClaytonBelinda Bullard

Abstract

Drosophila indirect flight muscles (IFMs) contain a 35 kDa protein which cross-reacts with antibodies to the IFM specific protein troponin-H isoform 34 (TnH-34). Peptide fingerprinting and peptide sequencing showed that this 35 kDa protein is glutathione S-transferase-2 (GST-2). GST-2 is present in the asynchronous indirect flight muscles but not in the synchronous tergal depressor of the trochanter (jump muscle). Genetic dissection of the sarcomere showed that GST-2 is stably associated with the thin filaments but the presence of myosin is required to achieve the correct stoichiometry, suggesting that there is also an interaction with the thick filament. The two Drosophila TnHs (isoforms 33 and 34) are naturally occurring fusion proteins in which a proline-rich extension of approximately 250 amino acids replaces the 27 C-terminal residues of the muscle-specific tropomyosin II isoform. The proteolytic enzyme, Igase, cleaves the hydrophobic C-terminal sequence of TnH-34 at three sites and TnH-33 at one site. This results in the release of GST-2 from the myofibril. The amount of GST-2 stably bound to the myofibril is directly proportional to the total amount of undigested TnH. It is concluded that GST-2 in the thin filament is st...Continue Reading

Citations

May 15, 2001·American Journal of Physiology. Cell Physiology·C A Conley
Apr 13, 2004·Journal of Cell Science·Upendra NongthombaJohn C Sparrow
Aug 25, 2017·American Journal of Physiology. Cell Physiology·Bernadette M GlasheenDouglas M Swank
Dec 10, 2019·The Journal of Cell Biology·Szilárd SzikoraJózsef Mihály
Sep 6, 2001·The Journal of Cell Biology·M KulkeW A Linke
May 23, 2003·Archives of Insect Biochemistry and Physiology·S ShenC Chien
Jun 6, 2006·Journal of Muscle Research and Cell Motility·Jesús MateosRoberto Marco
Oct 3, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·T S AwololaH Ranson
May 22, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Andor J KissRachael M Morgan-Kiss
Sep 25, 1999·The Journal of Biological Chemistry·A RazzaqJ C Sparrow
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alexander J WhitworthLeo J Pallanck
Sep 1, 2006·Journal of Bioenergetics and Biomembranes·Elayne Provost, Allen Shearn
Mar 24, 2011·Drug Metabolism Reviews·Jack U Flanagan, Mark L Smythe
Dec 18, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J O Vigoreaux
Feb 17, 2011·Biophysics·Hiroyuki Iwamoto
Feb 20, 2010·Insect Molecular Biology·J G OakeshottC Claudianos
Jun 4, 2018·Insect Biochemistry and Molecular Biology·Galit EakteimanShai Morin
Aug 16, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qiang WanJehee Lee
Nov 22, 2011·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ramavati PalAlan Clark

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.