PMID: 7085618Jun 25, 1982Paper

Interaction of tubulin with rat liver mitochondria.

The Journal of Biological Chemistry
F Bernier-Valentin, B Rousset

Abstract

Tubulin purified from rat brain was labeled by conjugation with N-succinimidyl 3-(4-hydroxy[5-125I]iodophenyl)propionate. Mitochondrial fraction prepared by centrifugation on sucrose density gradient was enriched about 4-fold in cytochrome c oxidase as compared to total liver homogenate. Contamination by plasma membranes was estimated to be about 5%. Radioiodinated pure tubulin bound to purified rat liver mitochondria; binding was time- and temperature-dependent: maximum binding was obtained after 45 min of incubation at 37 degrees C. Under conditions of binding, mitochondria retained their normal characteristics for phosphate accumulation. That binding actually occurs on mitochondria was demonstrated by the co-sedimentation of the tubulin binding and cytochrome c oxidase activities on sucrose gradient. Radioiodinated tubulin binding to mitochondria was specific and saturable. Saturation of binding was obtained using tubulin concentration ranging from 0.02 to 200 micrograms/ml. Hill plot and double reciprocal plot of binding data yielded values of 6 X 10(-8) M for an apparent KD and a maximal binding capacity of 1.4 nmol of tubulin/mg of mitochondrial protein. The Hill coefficient was 0.98 indicating that radioiodinated tubulin...Continue Reading

Related Concepts

Metazoa
Brain
Plasma Membrane
Cytoplasmic Matrix
Kinetics
Liver
Mitochondria, Liver
Plasma Protein Binding Capacity
Delta-Tubulin
Rattus

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