Interaction of vanadate and iodate oxyanions with adenylyl cyclase of ciliary processes

Biochemical Pharmacology
T W MittagT Taniguchi

Abstract

Vanadate (VO3-) was found to activate adenylyl cyclase (AC) in ocular ciliary process membrane. This response was additive to that of isoproterenol (ISO) and vasoactive intestinal peptide (VIP), but it was potentiative with forskolin (FSK) and also with Ca2+/calmodulin activation of AC activity. The potentiated response of FSK in the presence of VO3- was due to an increase in Vmax without a change in the apparent affinity of FSK or VO3-, and therefore differs from the potentiated response of activated G-protein (Gs) and FSK, where the affinity of FSK was increased by 1-2 orders of magnitude. Potentiation occurred at low Mg2+ and was not observed at free [Mg2+] > 3 mM. Iodate (IO3-) inhibited the FSK, ISO, and VO3- activations of AC in ciliary process membranes (IC50, 0.3 mM). In vivo topical treatment of the rabbit eye with 50 microL of 5% NaIO3 had no effect alone but completely blocked the intraocular pressure response to a 50-microL topical dose of 1% FSK and partially blocked the response to a 50-microL dose of 0.001% ISO. These findings indicate that some AC enzymes may have a binding site for oxyanions which can directly regulate enzyme activity.

References

Jan 11, 1979·Nature·U SchwabeE Söchtig
Jan 1, 1984·Annual Review of Pharmacology and Toxicology·B R Nechay
Aug 1, 1982·Biochemical and Biophysical Research Communications·G SwarupD L Garbers
Apr 1, 1984·Biochemical Pharmacology·E ErdmannH Scholz
Jan 1, 1981·Current Topics in Cellular Regulation·T Ramasarma, F L Crane

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Citations

Apr 18, 2001·Biological & Pharmaceutical Bulletin·T SuenagaH Ueki
Aug 23, 2003·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroshi Ueki
Oct 4, 2002·Archives of Biochemistry and Biophysics·Aya YoshidaHiroshi Ueki

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