PMID: 6985361Jun 1, 1980Paper

Interaction of wheat monomeric and dimeric protein inhibitors with alpha-amylase from yellow mealworm (Tenebrio molitor L. larva)

The Biochemical Journal
V BuonocoreV Silano

Abstract

The highly purified alpha-amylase from Tenebrio molitor L. larva (yellow mealworm) reversibly combines with two closely related homogeneous glycoprotein inhibitors, one dimeric (termed 'inhibitor 0.19') and one monomeric (termed 'inhibitor 0.28'), from wheat flour. As established by means of difference spectroscopy and kinetic studies, molar combining ratios for the amylase--inhibitor-0.19 and amylase-inhibitor-0.28 complexes were 1:1 and 1:2 respectively. Two amylase--inhibitor-0.19 complexes with slightly different retention volumes on Bio-Gel P-300 and only one amylase--inhibitor-0.28 complex were observed. Dissociation constants of the amylase--inhibitor-0.19 and amylase--inhibitor-0.28 complexes were 0.85 nM and 0.13 nM respectively. A strong tendency of both complexes to precipitate under an ultracentrifugal field was observed; the minimum molecular weight calculated for the two complexes under such conditions was approx. 95 000. The two complexes showed difference spectra indicating involvement of structurally related or identical tryptophyl side chains in the binding of inhibitors 0.28 and 0.19 to the amylase. A model summarizing the main features of the inhibition of the insect amylase by the two wheat protein inhibito...Continue Reading

Citations

May 24, 2008·Bioinorganic Chemistry and Applications·Zhu Zhi-FeiMa Yan-He
Feb 1, 1984·Journal of the Science of Food and Agriculture·V BuonocoreV Silano
Jun 20, 1998·Journal of Molecular Biology·S StroblR Glockshuber
Nov 10, 1993·Biochimica Et Biophysica Acta·S Alagiri, T P Singh
Feb 28, 1983·Biochimica Et Biophysica Acta·K MaedaH Matsubara
Jul 11, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Khaoula El HassouniC Friedrich H Longin
Mar 3, 2005·Journal of Agricultural and Food Chemistry·Octávio L FrancoMaria F Grossi-de-Sá

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