Interaction of Zn(2+) ions with single-stranded polyU and polyC in neutral solutions

The Journal of Physical Chemistry. B
V A SorokinValery Andrushchenko

Abstract

Effect of Zn(2+) ions on the conformation of single-stranded polynucleotides polyU and polyC in a wide temperature range at pH 7 was studied by differential UV spectroscopy and by thermal denaturation. The atoms coordinating Zn(2+) ions were determined (O4 and N3 in polyU and N3 in polyC). A three-dimensional phase diagram and its two-dimensional components were constructed for a polyC-Zn(2+) system. The phase diagram revealed a region in which ordered single-stranded structures, stabilized by Zn(2+)-mediated cross-links involving N3 atom of cytosine, are formed. The phase diagram also demonstrated that the behavior of the polyC-Zn(2+) system is similar to the effect of retrograde condensation observed in some binary solutions of simple liquids. A dependence of Zn(2+)-polyC binding constant on the metal ion concentration was obtained. The reason why zinc-induced transition of the sequences with adenine-uracil (AU) base pairs from A-form geometry to a metallized m-form requires higher pH compared to the sequences comprised of guanine-cytosine (GC) base pairs is explained. This information can be useful for the development of possible technological applications based on m-DNA.

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Nov 29, 2011·International Journal of Biological Macromolecules·V A SorokinV V Andrushchenko
Jan 15, 2013·International Journal of Biological Macromolecules·V A SorokinV V Andrushchenko
Aug 27, 2013·International Journal of Biological Macromolecules·V A SorokinV V Andrushchenko

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Citations

Sep 9, 2016·The Journal of Physical Chemistry. B·Ryan L MelvinFreddie R Salsbury

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