Interaction strengths between the ribosome and tRNA at various steps of translocation.

Biophysical Journal
Chen-Yu LiuTae-Hee Lee

Abstract

Transfer RNA (tRNA) translocates inside the ribosome during translation. We studied the interaction strengths between the ribosome and tRNA at various stages of translocation. We utilized an optical trap to measure the mechanical force to rupture tRNA from the ribosome. We measured the rupture forces of aminoacyl tRNA or peptidyl tRNA mimic from the ribosome in a prepeptidyl transfer state, the pretranslocational state, and the posttranslocational state. In addition, we measured the interaction strength between the ribosome and aminoacyl-tRNA in presence of viomycin. Based on the interaction strengths between the ribosome and tRNA under these conditions, 1), we concluded that tRNA interaction with the 30S subunit is far more important than the interaction with the 50S subunit in the mechanism of translocation; and 2), we propose a mechanism of translocation where the ribosomal ratchet motion, with the aid of EF-G, drives tRNA translocation.

References

Dec 1, 1977·European Journal of Biochemistry·J Modolell, Vázquez
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C G KurlandC Blomberg
Jun 3, 1986·Biochemistry·R LillW Wintermeyer
Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·A S Spirin
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·I Pettersson, C G Kurland
Dec 15, 1995·Journal of Molecular Biology·M O'Connor, A E Dahlberg
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·C BorowskiW Wintermeyer
Jan 1, 1997·Annual Review of Biochemistry·R Green, H F Noller
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R K AgrawalJ Frank
Jul 13, 2000·Journal of Molecular Biology·A SavelsberghW Wintermeyer
Nov 4, 2000·Nature Structural Biology·Y P SemenkovW Wintermeyer
May 8, 2001·Science·J M OgleV Ramakrishnan
May 9, 2001·Science·A E Dahlberg
Jun 8, 2001·Annual Review of Biochemistry·M V Rodnina, W Wintermeyer
Aug 21, 2001·Cell·G Z YusupovaH F Noller
Mar 23, 2002·Cell·V Ramakrishnan
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey L HansenThomas A Steitz
Oct 16, 2002·Nature Structural Biology·Holger StarkMarin van Heel
Jun 25, 2003·Molecular Cell·Andreas SavelsberghWolfgang Wintermeyer
Jul 16, 2003·Cell·Mikel ValleJoachim Frank
Oct 21, 2003·Nature Structural Biology·Mikel ValleJoachim Frank
Dec 3, 2004·Molecular Cell·Richard P FahlmanOlke C Uhlenbeck
Jun 14, 2005·Biophysical Journal·Francesco VanziYale E Goldman
Aug 1, 2006·The Review of Scientific Instruments·Keir C Neuman, Steven M Block
Sep 5, 2006·The Journal of Biological Chemistry·Taeko KomodaTsutomu Suzuki
Feb 24, 2007·Molecular Cell·Dongli PanBarry S Cooperman
May 22, 2007·Journal of Molecular Biology·Dmitri N ErmolenkoHarry F Noller
May 23, 2007·Nature Structural & Molecular Biology·Dmitri N ErmolenkoHarry F Noller
Jun 19, 2007·Current Opinion in Structural Biology·Veysel Berk, Jamie H D Cate
Aug 19, 2007·Biophysical Journal·Harold D KimSteven Chu
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tae-Hee LeeSteven Chu
Jun 10, 2008·Molecular Cell·Peter V CornishTaekjip Ha
Jan 9, 2009·Nucleic Acids Research·Taraka DaleOlke C Uhlenbeck
May 7, 2009·Nucleic Acids Research·Shinichiro ShojiKurt Fredrick
May 20, 2009·Journal of Molecular Biology·Jared M SchraderOlke C Uhlenbeck

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Citations

Jun 21, 2013·Nucleic Acids Research·Lin WangStephen J Benkovic
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi
Jun 12, 2014·Nature·Jin ChenJoseph D Puglisi

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