Interaction with antigen-specific T cells regulates expression of the lactate transporter MCT1 in primary rat astrocytes: specific link between immunity and homeostasis

Glia
Thomas KornStefan Jung

Abstract

Monocarboxylates like lactate are provided by astrocytes and can be used as fuel by neurons and oligodendrocytes. In an autoimmune inflammatory environment, homeostatic functions of astrocytes are incompletely understood. In primary Lewis rat astrocytes, co-culture with MHC class II-restricted myelin basic protein (MBP)-specific T cells in the presence of MBP resulted in a marked upregulation of the astrocytic lactate transporter MCT1 that is to export lactate into the extracellular space. It was evident that the increase in MCT1 was triggered by T cells in an antigen-dependent manner. The glial isoform of the glucose transporter GLUT1 was not regulated under these conditions. T-cell blasts that had been pre-activated by antigen and splenic antigen-presenting cells (APCs) beforehand also led to an increase in the expression of astrocytic MCT1 after co-culture. Resting T cells did not induce a relevant upregulation of MCT1 in astrocytes. However, resting T cells stimulated the expression of MCT1 when anti-MHC class II antibodies, but not when anti-MHC class I antibodies, were added to the co-culture. Therefore, even in the presence of inactive T cells, complexation of MHC class II molecules on astrocytes might lead to the regula...Continue Reading

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Citations

Jun 21, 2005·Journal of Cellular and Molecular Medicine·T Magnus, M S Rao
Aug 3, 2010·Biochimica Et Biophysica Acta·Smriti M Agrawal, V Wee Yong
Jan 16, 2008·Physiological Reviews·Tihomir Paul Obrenovitch
Aug 27, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas KornStefan Jung

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