Interaction with protocadherin-gamma regulates the cell surface expression of protocadherin-alpha

The Journal of Biological Chemistry
Yoji MurataTakeshi Yagi

Abstract

The protocadherin-alpha (CNR/Pcdhalpha) and protocadherin-gamma (Pcdhgamma) proteins, members of the cadherin superfamily, are putative cell recognition/adhesion molecules in the brain. Overexpressed cadherins are generally expressed on the cell surface and elicit cell adhesion activity in several cell lines, although hardly any overexpressed CNR/Pcdhalpha proteins are expressed on the cell surface, except on HEK293T cells, which show low expression. We analyzed the expression of CNR/Pcdhalpha and Pcdhgamma in HEK293T cells and found that they formed a protein complex and that Pcdhgamma enhanced the surface expression of CNR/Pcdhalpha. This enhanced surface expression was confirmed by flow cytometry analysis and by marking cell surface proteins with biotin. The enhancement was observed using different combinations of CNR/Pcdhalpha and Pcdhgamma proteins. The surface expression activity was enhanced by the extracellular domains of the proteins, which could bind each other. Their cytoplasmic domains also had binding activity and influenced their localization. Their protein-protein interaction was also detected in extracts of mouse brain and two neuroblastoma cell lines. Thus, interactions between CNR/Pcdhalpha and Pcdhgamma regul...Continue Reading

References

Feb 9, 2000·The Journal of Cell Biology·W S ShanL Shapiro
Jul 19, 2002·Neuron·Hideru TogashiMasatoshi Takeichi
Dec 7, 2002·Neuron·Xiaozhong WangJoshua R Sanes
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Lars Ellgaard, Ari Helenius

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Citations

Dec 13, 2005·Journal of Molecular Evolution·Leonard LipovichEdison T Liu
Jul 21, 2006·Cell and Tissue Research·Kerstin PiechottaMarkus Missler
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dietmar Schreiner, Joshua A Weiner
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stefanie S SchalmTom Maniatis
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sean M BuchananTom Maniatis
Dec 3, 2008·The Journal of Biological Chemistry·Jian ChenXiaozhong Wang
Oct 22, 2009·Molecular & Cellular Proteomics : MCP·Meng-Hsuan HanXiaozhong Wang
Jun 26, 2012·Journal of Molecular Cell Biology·Lun SuoQiang Wu
Jan 3, 2012·Nucleic Acids Research·Michal Golan-MashiachEhud Shapiro
Apr 24, 2008·Development, Growth & Differentiation·Takeshi Yagi
Apr 4, 2007·Molecular and Cellular Biology·Stefan BonnMartin K Schwarz
May 6, 2014·Trends in Cell Biology·Marcos SotomayorDavid P Corey
Jul 15, 2015·Critical Reviews in Biochemistry and Molecular Biology·Shabnam MassahGratien G Prefontaine
Apr 15, 2015·Cell Adhesion & Migration·Austin B KeelerJoshua A Weiner
Jul 3, 2013·Journal of Neurogenetics·Takeshi Yagi
Nov 22, 2014·Journal of Biochemistry·Yusuke IzutaShintaro T Suzuki
Jan 18, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Christina DillingMalgorzata Burek
Nov 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kerry M GoodmanLawrence Shapiro
Oct 9, 2018·Annual Review of Cell and Developmental Biology·George MountoufarisTom Maniatis
May 10, 2007·Nature Protocols·Shigeyuki EsumiTakeshi Yagi
Jul 1, 2010·The Journal of Comparative Neurology·Yanfang LiAngel L De Blas
Sep 21, 2010·The European Journal of Neuroscience·Mónica Fernández-MonrealGreg R Phillips
Jan 13, 2006·Journal of Neurobiology·Gallen B Triana-Baltzer, Martina Blank
Apr 27, 2012·Physiological Reviews·Shinji Hirano, Masatoshi Takeichi
Apr 8, 2015·Journal of Neuroendocrinology·G M Coughlin, D M Kurrasch
Oct 15, 2019·The Journal of Comparative Neurology·Celia P MirallesAngel L De Blas
Jun 21, 2017·Cellular and Molecular Life Sciences : CMLS·Stacey L PeekJoshua A Weiner
Jul 11, 2019·Clinical Epigenetics·Ana Florencia Vega-BenedettiPatrizia Zavattari
Apr 12, 2011·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Marsha D Speevak, Sandra A Farrell
May 10, 2017·Frontiers in Molecular Neuroscience·Sonoko HasegawaTakeshi Yagi
Sep 18, 2020·Neuroscience Bulletin·Qiang Wu, Zhilian Jia
Sep 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Fengyan ZhouYongfeng Jin
Jul 28, 2019·Neuromolecular Medicine·Yan Jun Lee, Toh Hean Ch'ng

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