Interaction with Suv39H1 is critical for Snail-mediated E-cadherin repression in breast cancer.

Oncogene
C DongB P Zhou

Abstract

Expression of E-cadherin, a hallmark of epithelial-mesenchymal transition (EMT), is often lost due to promoter DNA methylation in basal-like breast cancer (BLBC), which contributes to the metastatic advantage of this disease; however, the underlying mechanism remains unclear. Here, we identified that Snail interacted with Suv39H1 (suppressor of variegation 3-9 homolog 1), a major methyltransferase responsible for H3K9me3 that intimately links to DNA methylation. We demonstrated that the SNAG domain of Snail and the SET domain of Suv39H1 were required for their mutual interactions. We found that H3K9me3 and DNA methylation on the E-cadherin promoter were higher in BLBC cell lines. We showed that Snail interacted with Suv39H1 and recruited it to the E-cadherin promoter for transcriptional repression. Knockdown of Suv39H1 restored E-cadherin expression by blocking H3K9me3 and DNA methylation and resulted in the inhibition of cell migration, invasion and metastasis of BLBC. Our study not only reveals a critical mechanism underlying the epigenetic regulation of EMT, but also paves a way for the development of new treatment strategies against this disease.

References

Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Feb 13, 2002·Current Opinion in Structural Biology·Warren L DeLano
May 8, 2002·Nature Reviews. Molecular Cell Biology·M Angela Nieto
Dec 24, 2003·Molecular Cell·Antoine H F M PetersThomas Jenuwein
Mar 3, 2004·Molecular and Cellular Biology·Jonathan E DodgeEn Li
Jun 3, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Weiya XiaMien-Chie Hung
Aug 10, 2005·Genome Biology·Shane C DillonXiaodong Cheng
Aug 16, 2005·Current Opinion in Genetics & Development·François Fuks
Sep 20, 2005·Cancer Cell·Susan E MoodyLewis A Chodosh
Nov 2, 2005·Nature Reviews. Molecular Cell Biology·Cyrus Martin, Yi Zhang
Jan 13, 2006·Nature Chemical Biology·Dorothea GreinerAxel Imhof
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Jean Paul Thiery, Jonathan P Sleeman
Jan 16, 2007·Molecular Cell·Alexander J RuthenburgJoanna Wysocka
Feb 27, 2007·Cell·Peter A Jones, Stephen B Baylin
Feb 27, 2007·Cell·Tony Kouzarides
May 18, 2007·Nature Reviews. Cancer·Héctor PeinadoAmparo Cano
May 25, 2007·Nature·Andrew P Feinberg
Nov 2, 2007·The Journal of Pathology·G StorciM Bonafé
Feb 19, 2008·Cancer Research·David SarrióJosé Palacios
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Mar 18, 2008·Trends in Biochemical Sciences·Federico FornerisAndrea Mattevi
Apr 23, 2008·Current Protocols in Bioinformatics·Narayanan EswarAndrej Sali
May 1, 2008·Journal of the National Cancer Institute·Xiaoxian LiJenny C Chang
May 8, 2008·Clinical & Experimental Metastasis·T BlickE W Thompson
Jun 10, 2008·Developmental Cell·Jing Yang, Robert A Weinberg
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·Yadi Wu, Binhua P Zhou
Nov 14, 2008·The Journal of Biological Chemistry·Yadi WuBinhua P Zhou
Mar 6, 2009·Nature Reviews. Cancer·Kornelia Polyak, Robert A Weinberg
Mar 25, 2009·Nature Reviews. Genetics·Howard Cedar, Yehudit Bergman
May 12, 2009·Trends in Genetics : TIG·Alejandro Barrallo-Gimeno, M Angela Nieto
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jun 11, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael T McCabePaula M Vertino
Jul 10, 2009·Protein Engineering, Design & Selection : PEDS·M S MadhusudhanAndrej Sali

❮ Previous
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Citations

Nov 10, 2013·Nature Medicine·Wai Leong Tam, Robert A Weinberg
Feb 14, 2016·Current Opinion in Genetics & Development·Alexandra Avgustinova, Salvador Aznar Benitah
Feb 26, 2016·Molecular Cancer·Silvia Juliana Serrano-GomezSuresh K Alahari
Sep 24, 2015·Cellular and Molecular Life Sciences : CMLS·Matjaz RokavecHeiko Hermeking
Aug 3, 2014·Journal of Experimental & Clinical Cancer Research : CR·Samantha Kaufhold, Benjamin Bonavida
Dec 4, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Horacio CardenasDaniela Matei
Apr 29, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Annina Nickel, Sonja C Stadler
Jan 7, 2015·Journal of Zhejiang University. Science. B·Hui-xin LiuChen-fang Dong
Dec 9, 2014·Cell Adhesion & Migration·E-Jean TanAristidis Moustakas
Dec 9, 2014·Cell Adhesion & Migration·Sarah Phillips, Charlotte Kuperwasser
May 23, 2014·International Journal of Cancer. Journal International Du Cancer·Tetsuhiro ChibaOsamu Yokosuka
May 31, 2015·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Nastaran Mohammadi Ghahhari, Sadegh Babashah
May 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tong GaoLian-Hong Li
Jul 9, 2013·Cancer Treatment Reviews·Pasano Bojang, Kenneth S Ramos
Dec 10, 2014·Medical Oncology·Chun-Xiong ZhaoXiao-Hou Wu
Jan 29, 2014·Oncogene·M F MontenegroJ N Rodríguez-López
Apr 15, 2016·Journal of Clinical Medicine·Trung VuPran K Datta
Jul 2, 2016·Cell·M Angela NietoJean Paul Thiery
Oct 23, 2016·Cellular and Molecular Life Sciences : CMLS·Lidong Sun, Jia Fang
Nov 12, 2016·Cancer Gene Therapy·E FaghihlooT Mokhtari-Azad
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
May 15, 2013·Molecular & Cellular Proteomics : MCP·Chen DingJun Qin
Jul 13, 2017·Epigenomics·Amir Hosseini, Saverio Minucci
May 8, 2018·Molecular Oncology·Varun MaturiAristidis Moustakas
Jan 31, 2018·BioMed Research International·Shuai WangYu Ling Sun
Aug 17, 2018·International Journal of Molecular Sciences·Armando CeveniniEsther Imperlini
Sep 20, 2017·Molecular and Cellular Biology·Sayyed K ZaidiGary S Stein
Jul 24, 2013·Molecular and Cellular Biology·Cheng ChangArthur M Mercurio
Dec 13, 2017·The Journal of Cell Biology·Manuel ViottiIra Mellman

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Methods Mentioned

BETA
co-immunoprecipitation
Co-IP
acetylation
ChIP
PCR
xenograft
transfection
Assay
Immunoprecipitation

Software Mentioned

MODELLER

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