PMID: 11341372May 9, 2001Paper

Interactions among proteins and hydrophobically modified polyelectrolytes

The Journal of Pharmacy and Pharmacology
Lev Bromberg

Abstract

A special class of hydrophobically modified polyelectrolytes was studied wherein poly(acrylic acid) (PAA) was conjugated with Pluronic F127 NF surfactant. The Pluronic-PAA copolymer solutions form gels at low concentrations when exposed to bodytemperature. Such gels possess enhanced retention in topical applications. Circular dichroism spectra indicate that tertiary structures of human insulin, haemoglobin, and albumin were stabilized in solutions of Pluronic-PAA. Aggregation of insulin in gelled solutions of Pluronic-PAA was impeded as demonstrated in shaking tests. The presence of Pluronic-PAA hindered the insulin degradation by alpha-chymotrypsin by at least 7-fold. Extraction of calcium ions from trypsin by Pluronic-PAA led to the dramatic changes in the tertiary structure and total loss of enzymatic activity, suggesting that Pluronic-PAA could inhibit tryptic degradation of proteins.

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Citations

Nov 11, 2006·Pharmaceutical Research·Gilles DumortierJean Claude Chaumeil
Sep 27, 2007·Pharmaceutical Research·Martin Werle
Feb 15, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lev Bromberg, Valery Alakhov
Oct 10, 2006·AAPS PharmSciTech·Rita J MajithiyaRayasa S R Murthy
Feb 27, 2015·Pharmaceutical Development and Technology·Milica Vukićević, Hanne Hjorth Tønnesen
Nov 22, 2005·Expert Opinion on Drug Delivery·Lev Bromberg
Mar 8, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lev Bromberg
Oct 27, 2007·International Journal of Pharmaceutics·Wen-Di MaWei-San Pan
Sep 1, 2004·International Journal of Pharmaceutics·Lev BrombergT Alan Hatton
Nov 15, 2013·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Vinod L Gaikwad, Manish S Bhatia
Sep 11, 2013·The Journal of Physical Chemistry. B·Bimlesh OjhaDeguo Du

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