Interactions between chensinin-1, a natural antimicrobial peptide derived from Rana chensinensis, and lipopolysaccharide

Biopolymers
Weibing DongDejing Shang

Abstract

Lipopolysaccharide (LPS) plays a critical role in the pathogenesis of sepsis caused by gram-negative bacterial infections. Therefore, LPS-neutralizing molecules would have important clinical applications. Chensinin-1, a novel antimicrobial peptide with atypical structural features, was found in the skin secretions of the Chinese brown frog Rana chensinensis. To understand the role of LPS in the bacterial susceptibility to chensinin-1 and to investigate its anti-endotoxin effects, the interactions of chensinin-1 with LPS were investigated in this study using circular dichroism, in situ IR, isothermal titration calorimetry, and zeta potential. This study is the first to use in situ IR spectroscopy to evaluate the secondary structural changes of this peptide. The capacity of chensinin-1 to block the LPS-dependent cytokine secretion of macrophages was also investigated. Our results show that chensinin-1 can form α-helical structures in LPS suspensions. LPS can affect the antimicrobial activity of chensinin-1, and chensinin-1 was able to mitigate the effects of LPS. These data may facilitate the development of antimicrobial peptides with potent antimicrobial and anti-endotoxin activities.

References

Jan 1, 1995·Annual Review of Immunology·H G Boman
Nov 4, 2000·Expert Opinion on Investigational Drugs·R E Hancock
Dec 26, 2001·Antimicrobial Agents and Chemotherapy·Oscar CirioniGiorgio Scalise
Feb 18, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Isaac Ginsburg
Oct 13, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Valery V AndrushchenkoElmar J Prenner
Jun 9, 2007·Biochimica Et Biophysica Acta·Xi ChenKlaus Brandenburg
Jun 13, 2009·The Journal of Biological Chemistry·Anirban BhuniaSurajit Bhattacharjya
May 21, 2011·Cellular and Molecular Life Sciences : CMLS·Andrea Giuliani, Andrea C Rinaldi
Nov 15, 2011·Critical Reviews in Biotechnology·Mukesh PasupuletiMartin Malmsten
Dec 14, 2011·The Journal of Investigative Dermatology·Teruaki Nakatsuji, Richard L Gallo
Dec 17, 2011·Nature Reviews. Drug Discovery·Christopher D FjellGisbert Schneider

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