Interactions between coat and scaffolding proteins of phage P22 are altered in vitro by amino acid substitutions in coat protein that cause a cold-sensitive phenotype

Biochemistry
C M Teschke, D G Fong

Abstract

Cold-sensitive mutations in phage P22 coat protein cause the accumulation of precursor capsids in cells growing at the nonpermissive temperature (16 degrees C). The assembly of coat proteins which carry the substitutions threonine at position 10 to isoluecine (T10I), arginine at position 101 to cysteine (R101C), or asparagine at position 414 to serine (N414S) which cause cold-sensitivity has been investigated. All three proteins were found to fold into a monomeric species. Coat proteins carrying the amino acid substitutions T10I and R101C were not able to interact with scaffolding protein appropriately to initiate assembly in vitro while coat protein carrying the substitution N414S was able to assemble; however, capsids formed of this protein had an increased affinity for scaffolding protein. These amino acid substitutions define two regions in coat protein that are essential for the interaction of coat protein with scaffolding protein at different stages in capsid maturation.

References

Jun 15, 1979·Journal of Molecular Biology·S Casjens
Aug 1, 1976·Journal of Virology·B EdvardssonL Philipson
May 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Seckler, R Jaenicke
Jul 5, 1988·Journal of Molecular Biology·C Bazinet, J King
Sep 1, 1995·Journal of Bacteriology·M J Lelivelt, T H Kawula
Jul 1, 1993·Biophysical Journal·M L Galisteo, J King

❮ Previous
Next ❯

Citations

Feb 15, 2011·Archives of Virology·Young-Duck LeeJong-Hyun Park
Aug 8, 2007·Molecular Microbiology·Kristin N ParentCarolyn M Teschke
Oct 9, 1998·The Journal of Biological Chemistry·W S Nakonechny, C M Teschke
Apr 17, 2001·The Journal of Biological Chemistry·L A Aramli, C M Teschke
Jul 29, 1998·Journal of Molecular Biology·M H ParkerP E Prevelige

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.