PMID: 9163913May 1, 1997Paper

Interactions between Fusobacterium necrophorum hemolysin, erythrocytes and erythrocyte membranes

FEMS Microbiology Letters
K K AmoakoT Shinjo

Abstract

The interactions between the hemolysin of Fusobacterium necrophorum subsp. necrophorum, erythrocytes and erythrocyte membranes were studied as an attempt to determine the initial characteristics leading to hemolysis. The spectrum of erythrocyte sensitivity indicated that horse, dog and mouse erythrocytes were highly sensitive whereas those of cattle, sheep, goat and chicken were insensitive to the hemolysin. Binding of hemolysin to horse and dog erythrocytes or their ghosts was more pronounced than to those of cattle and sheep as detected by a decrease of hemolytic activity from hemolysin preparations. The kinetics of hemolysis revealed that lysis is preceded by a prelytic phase characterized by binding of hemolysin to erythrocytes. Treatment of horse erythrocytes with hemolysin at various temperatures prior to incubation at 37 degrees C also revealed that this binding prelytic phase is temperature independent. This was followed by a temperature dependent lytic stage since erythrocytes pretreated with hemolysin and incubated at 4 degrees C showed no hemolysis. An inverse relation was found between erythrocyte concentration and hemolytic activity suggesting a multiple-hit mechanism of hemolysis.

References

Oct 1, 1977·Canadian Journal of Microbiology·M M GarciaK A McKay
Feb 18, 1972·Science·S J Singer, G L Nicolson
Jul 1, 1994·Veterinary Microbiology·K K AmoakoT Shinjo
May 1, 1996·Veterinary Microbiology·K K AmoakoT Shinjo

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Citations

Nov 8, 2006·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·A S GoriunovS P Rozhkov
Aug 30, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Hui LvJin-Ku Bao
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C O EnwonwuE O Idigbe

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