PMID: 2503252Aug 11, 1989Paper

Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence

Cell
C MurreA B Lassar

Abstract

A DNA binding and dimerization motif, with apparent amphipathic helices (the HLH motif), has recently been identified in various proteins, including two that bind to immunoglobulin enhancers (E12 and E47). We show here that various HLH proteins can bind as apparent heterodimers to a single DNA motif and also, albeit usually more weakly, as apparent homodimers. The HLH domain can mediate heterodimer formation between either daughterless, E12, or E47 (Class A) and achaete-scute T3 or MyoD (Class B) to form proteins with high affinity for the kappa E2 site in the immunoglobulin kappa chain enhancer. The achaete-scute T3 and MyoD proteins do not form kappa E2-binding heterodimers together, and no active complex with N-myc was evident. The formation of a heterodimer between the daughterless and achaete-scute T3 products may explain the similar phenotypes of mutants at these two loci and the genetic interactions between them. A role of E12 and E47 in mammalian development, analogous to that of daughterless in Drosophila, is likely.

References

Dec 1, 1988·Genes & Development·F J RauscherT Curran
Dec 15, 1988·Nature·T Kouzarides, E Ziff
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·F W AltP Nisen
Dec 1, 1988·Genes & Development·R G ClercP A Sharp
Dec 1, 1988·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Christine Dambly-ChaudièreYuh Nung Jan

❮ Previous
Next ❯

Citations

Nov 26, 1999·Journal of Cellular Biochemistry·M S LeeC A Glackin
Jan 1, 1996·Developmental Genetics·B Hassan, H Vaessin
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G L Blatch, M Lässle
Jan 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·E M Füchtbauer, H Westphal
Mar 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A FaermanM Shani
Jan 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H G SimonC Tabin
Mar 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K KoishiA J Harris
Oct 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Garrell, S Campuzano
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M A Rudnicki, R Jaenisch
Dec 14, 2004·Chembiochem : a European Journal of Chemical Biology·Amy C BeltranJoel M Gottesfeld
Apr 1, 1995·Microscopy Research and Technique·M OntellM Buckingham
Jul 1, 1990·Molecular Reproduction and Development·M B Dworkin, E Dworkin-Rastl
Sep 1, 1992·International Journal of Cell Cloning·A R Green, C G Begley
Sep 1, 1996·Somatic Cell and Molecular Genetics·D K Rohrer, H M Blau
Apr 1, 1995·Molecular Neurobiology·J A Campos-Ortega
Mar 13, 2002·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Nadean L BrownTom Glaser
Mar 23, 2006·Archives of Dermatological Research·Elisabeth Björntorp MarkAnders Lindahl
May 4, 2007·Molecular Genetics and Genomics : MGG·Kellie E BarbaraGeorge M Santangelo
Nov 7, 2008·Molecular Genetics and Genomics : MGG·Zhen-Xing JiJu-Hua Ni
Feb 22, 2011·Medical Oncology·Liu XinghuaChen Jianying
Aug 18, 2012·Virologica Sinica·Xiuning SunZhijun Liu
Mar 1, 1992·Developmental Biology·G E Kovalick, K Beckingham
May 1, 1992·Developmental Biology·C G Jennings
Feb 1, 1992·The International Journal of Biochemistry·J E Hesketh, P F Whitelaw
Dec 1, 1992·The International Journal of Biochemistry·W M Krajewska
Dec 1, 1995·Leukemia Research·A IshiguroH P Koeffler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.