PMID: 108802Jan 1, 1979

Interactions between mouse immunoglobulins and staphylococcal protein A

Scandinavian Journal of Immunology
M P ChalonJ P Vaerman

Abstract

When mouse serum or ascites is applied on protein A-Sepharose columns and washed with enough phosphate-buffered saline, a second protein peak is often eluted with the same buffer after the first major peak of unbound proteins. This second peak is almost pure Ig G1. More IgG1 plus IgG2a, IgG2b and IgG3 are thereafter eluted with acid saline. 90% of the IgG1 whichhad been eluted with neutral buffer could be re-eluted at the same retarded position with the same buffer. When a gradient from 0 to 3 M sodium thiocyanate was started after the major peak of unbound proteins, all IgG1 was eluted before IgG2 and IgG3. These results suggest that IgG1 has a much lower affinity for protein A than IgG2 or IgG3 and that normal mouse serum IgG1 can be purified by such a simple procedure.

References

Dec 1, 1976·Immunochemistry·M Stanislawski, M Mitard
Jan 1, 1978·Scandinavian Journal of Immunology·J GoudswaardJ P Vaerman
Jan 1, 1978·Journal of Immunological Methods·J W Goding
Jan 1, 1975·Immunochemistry·S Dissanayake, F C Hay
Jan 1, 1974·Scandinavian Journal of Immunology·M Harboe, I Folling

Citations

Apr 1, 1995·Endocrine Pathology·Carin BacklinJ Rastad
Apr 22, 1988·Journal of Immunological Methods·Y Y TuD M Goldenberg
Jul 1, 1981·Molecular Immunology·J RousseauxG Biserte
Mar 1, 1985·Molecular Immunology·D R Burton
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L A SternbergerN H Sternberger
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C JuhlinL Klareskog
Oct 1, 1981·Scandinavian Journal of Immunology·I J SeppäläO Mäkelä
Mar 1, 1982·Scandinavian Journal of Immunology·E Van LoghemE C Franklin
Jun 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·G Marazzi, K M Buckley
Oct 24, 2019·Protein Engineering, Design & Selection : PEDS·Ohan MazigiDaniel Christ

Related Concepts

Ascites
Chromatography, Affinity
Electrophoresis, Agar Gel
Immunodiffusion Measurement
IgA2
Polyglobin
IgM2
Immune Sera
Immunoelectrophoresis
Antibody Fragments

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