PMID: 6169799Jan 1, 1981Paper

Interactions between polymerized human albumin, hepatitis B surface antigen, and complement: I. Binding of polyalbumin to Clq

Journal of Medical Virology
D R MilichG N Vyas

Abstract

There is considerable evidence that substances other than immunoglobulins can bind human Clq. Utilizing purified human Clq immobilized on polystyrene beads, we have demonstrated that polymerized human albumin (PHALB-125I) binds to human Clq in a direct binding assay. This interaction required a high degree of albumin polymerization as the percentage of binding was proportional to the polymer size and monomeric albumin was unreactive. Binding was species specific in the human Clq bound only human, and not xenogeneic, polyalbumins. Similarly, polymers of various other human plasma proteins were unreactive. To demonstrate that this interaction was not unique to immobilized Clq, soluble Clq was shown to inhibit PHALB-125I binding to solid phase Clq. Because aggregated IgG, poly(I):poly(C), dextran sulfate, polyglutamic acid, and polylysine have been previously shown to bind Clq, we used them in further blocking experiments and found them also to inhibit the interaction between Clq and PHALB. Anti-human Clq and, to a lesser extent, anti-PHALB antibodies inhibited the interaction. The Clq-PHALB binding was pH, ionic strength, and temperature dependent. In addition, human Clq was not observed to bind hepatitis B surface antigen (HBsAg...Continue Reading

References

Feb 1, 1978·Immunochemistry·T Y Lin, D S Fletcher
May 1, 1975·The Journal of General Virology·C J Burrell
Dec 15, 1977·Nature·N C Hughes-JonesJ Rowlands
Jan 1, 1977·Journal of Medical Virology·R LenkeiI Dobre
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A R NeurathA Lippin
Aug 1, 1970·British Journal of Pharmacology·V Eisen, C Loveday

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Citations

Jun 10, 1986·Journal of Immunological Methods·D E SansonnoO G Manghisi
Feb 5, 2002·Biochimica Et Biophysica Acta·Sandor SavayCarl R Alving
Jan 1, 1983·Journal of Medical Virology·M TotiF Bonino
Apr 1, 1985·Journal of Medical Virology·B K GiljaM A Gerber
Mar 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A AlbertiG Realdi

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