Interactions between protein coated particles and polymer surfaces studied with the rotating particles probe

Langmuir : the ACS Journal of Surfaces and Colloids
M KemperM W J Prins

Abstract

Nonspecific interactions between proteins and polymer surfaces have to be minimized in order to control the performance of biosensors based on immunoassays with particle labels. In this paper we investigate these nonspecific interactions by analyzing the response of protein coated magnetic particles to a rotating magnetic field while the particles are in nanometer vicinity to a polymer surface. We use the fraction of nonrotating (bound) particles as a probe for the interaction between the particles and the surface. As a model system, we study the interaction of myoglobin coated particles with oxidized polystyrene surfaces. We measure the interaction as a function of the ionic strength of the solution, varying the oxidation time of the polystyrene and the pH of the solution. To describe the data we propose a model in which particles bind to the polymer by crossing an energy barrier. The height of this barrier depends on the ionic strength of the solution and two interaction parameters. The fraction of nonrotating particles as a function of ionic strength shows a characteristic shape that can be explained with a normal distribution of energy barrier heights. This method to determine interaction parameters paves the way for furthe...Continue Reading

References

Jan 5, 2002·Quarterly Reviews of Biophysics·D Leckband, J Israelachvili
Oct 24, 2008·Biosensors & Bioelectronics·Farzana DarainSwee Chuan Tjin
Mar 6, 2009·Biosensors & Bioelectronics·V C MartinsL P Fonseca
Oct 5, 2010·Colloids and Surfaces. B, Biointerfaces·Dong-Hao ZhangYu-Lei Xie
May 5, 2011·Biophysical Journal·X J A JanssenM W J Prins
May 11, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Lei Shen, X-Y Zhu
Jun 28, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Stela Andrea MunteanAlexey V Lyulin

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Citations

Sep 21, 2012·Analytical Chemistry·Asha JacobMenno W J Prins
Oct 15, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·M R W ScheepersM W J Prins

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