PMID: 7030618Jan 1, 1981Paper

Interactions between soluble enzymes and subcellular structure

CRC Critical Reviews in Biochemistry
C J Masters

Abstract

Soluble enzymes contribute significantly to the metabolic capabilities of living organisms, but it is becoming increasingly clear that the activities of these enzymes are significantly modified by their interactions with structural components of the cells, and that these interactions may make important contributions to metabolic regulation. In the past, specification of these interactions has been limited by the availability of suitable experimental techniques, but this deficiency is now being rectified and our understanding of these processes if advancing rapidly. Research in this area is moving into a second phase, with the emphasis no longer being focused on demonstrations of the biological reality of these interactions, but directed more towards quantitative aspects of binding, the determination of the characteristics of binding domains, and the theoretical basis of regulatory involvements. All of these aspects are discussed in the present review.

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