Abstract
The N-ethylmaleimide reactivity of c subunits in Escherichia coli F1F0 ATP synthase (ECF1F0) isolated from five mutants, each with a cysteine at a different position in the polar loop region (positions 39, 40, 42, 43, and 44), has been investigated. The maleimide was found to react with Cys placed at positions 42, 43, and 44 but not at 39 or 40. All copies of the c subunit reacted similarly when the Cys was at position 43 or 44. In contrast, the Cys in the mutant cQ42C reacted as two classes, with 60% reacting relatively rapidly and 40% reacting at a rate 40-fold slower. After removing F1, all copies of the c subunit in this mutant reacted equally fast. Therefore, the slow class in the cQ42C mutant represents c subunits shielded by, and probably involved directly in, the interaction of the F0 with gamma and epsilon subunits of the F1 part. Based on the estimated stoichiometry of c subunits in the ECF1F0 complex, 4 or 5 c subunits are involved in this F1 interaction. N-Ethylmaleimide modification of all of the c subunits reduced ATPase activity by only 30% in ECF1F0 from mutant cQ42C. Modification of the more rapidly reacting class had little effect on ATP hydrolysis-driven proton translocation, and did not alter the DCCD inhibi...Continue Reading
References
Jun 29, 1976·Biochemistry·N W Downer, N C Robinson
Oct 1, 1992·Journal of Bioenergetics and Biomembranes·R H Fillingame
Feb 5, 1991·Biochemistry·J Mendel-Hartvig, R A Capaldi
Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·A E Senior
Jan 1, 1988·Physiological Reviews·A E Senior
Nov 3, 1987·Biochemistry·R AggelerR A Capaldi
Aug 15, 1970·Nature·U K Laemmli
Apr 9, 1984·Biochimica Et Biophysica Acta·J Hoppe, W Sebald
Aug 28, 1984·Biochemistry·H R LötscherR A Capaldi
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·T M DuncanR L Cross
Nov 1, 1995·Nature Structural Biology·S WilkensR A Capaldi
Oct 13, 1995·The Journal of Biological Chemistry·Y Zhang, R H Fillingame
Jun 1, 1995·Biochimica Et Biophysica Acta·R AggelerR A Capaldi
Jul 17, 1995·FEBS Letters·S D WattsR A Capaldi
Feb 7, 1995·Biochemistry·M E Girvin, R H Fillingame
Nov 29, 1994·Biochemistry·P Turina, R A Capaldi
Jan 1, 1994·Biological Chemistry Hoppe-Seyler·S Wilkens, R A Capaldi
Jul 1, 1994·Trends in Biochemical Sciences·R A CapaldiS Wilkens
Aug 25, 1994·Nature·J P AbrahamsJ E Walker
Jun 6, 1994·FEBS Letters·J E Walker, I R Collinson
Nov 16, 1993·Biochemistry·M E Girvin, R H Fillingame
Jan 25, 1994·Biochemistry·M E Girvin, R H Fillingame
Jan 8, 1993·Biochimica Et Biophysica Acta·P D Boyer
Feb 9, 1996·The Journal of Biological Chemistry·C Tang, R A Capaldi
Jun 13, 1996·Nature·D SabbertW Junge
Jun 7, 1996·The Journal of Biological Chemistry·R Aggeler, R A Capaldi
Aug 1, 1996·Current Opinion in Structural Biology·R H Fillingame
Nov 8, 1996·The Journal of Biological Chemistry·S D WattsR A Capaldi
Nov 11, 1996·FEBS Letters·S SinghR Capaldi
Oct 1, 1996·Journal of Bioenergetics and Biomembranes·R A CapaldiG Grüber
Dec 20, 1996·The Journal of Biological Chemistry·H LillS Engelbrecht
Mar 1, 1997·Nature Structural Biology·S WilkensR A Capaldi
Citations
Jun 6, 2000·Biochimica Et Biophysica Acta·J VeloursP V Graves
Dec 15, 2000·Current Opinion in Structural Biology·D StockJ E Walker
Feb 24, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Z Li, G J Neufeld
Sep 22, 1999·European Journal of Biochemistry·R BirkenhägerG Deckers-Hebestreit
Feb 20, 2003·Journal of Bacteriology·Wendi L Kuhnert, Robert G Quivey
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·R K NakamotoM K al-Shawi
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·M L HutcheonR L Cross
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·W Junge
Oct 16, 1999·The Journal of Biological Chemistry·M RevingtonS D Dunn
Jun 8, 1999·The Journal of Biological Chemistry·J HermolinR H Fillingame
Aug 26, 1998·The Journal of Biological Chemistry·C J Ketchum, R K Nakamoto
Oct 10, 1997·FEBS Letters·S Engelbrecht, W Junge
Feb 7, 2001·The Journal of Biological Chemistry·P C JonesR H Fillingame
Oct 29, 1998·The Journal of Biological Chemistry·A J Rodgers, R A Capaldi
Nov 24, 1999·The Journal of Biological Chemistry·B SchulenbergR A Capaldi
Jul 21, 2004·The Journal of Biological Chemistry·Sam M MazhariChristine R Cremo
Apr 16, 2002·European Journal of Biochemistry·Ulrich MattheyPeter Dimroth
Jun 11, 2008·Biochemistry·Thomas KrebstakiesGabriele Deckers-Hebestreit
Apr 5, 2000·Biochemistry·P PaumardJ Velours
Jan 12, 1999·Archives of Biochemistry and Biophysics·J L Gardner, B D Cain
Nov 7, 1998·Biochimica Et Biophysica Acta·C SpannagelJ Velours
Sep 27, 2000·Biochimica Et Biophysica Acta·Y WadaM Futai
Jun 6, 2000·Biochimica Et Biophysica Acta·G Deckers-HebestreitK Altendorf
Jun 6, 2000·Biochimica Et Biophysica Acta·S B VikD Zhang
Apr 1, 2007·EcoSal Plus·Steven B Vik
Nov 11, 1999·Biochemistry·V SoubannierJ Velours
Sep 20, 2000·Biochemistry·Y B Peskova, R K Nakamoto
Nov 18, 1998·Biochemistry·H XiongS B Vik