Interactions between the Powdery Mildew Effector BEC1054 and Barley Proteins Identify Candidate Host Targets.

Journal of Proteome Research
Helen G PenningtonLaurence V Bindschedler

Abstract

There are over 500 candidate secreted effector proteins (CSEPs) or Blumeria effector candidates (BECs) specific to the barley powdery mildew pathogen Blumeria graminis f.sp. hordei. The CSEP/BEC proteins are expressed and predicted to be secreted by biotrophic feeding structures called haustoria. Eight BECs are required for the formation of functional haustoria. These include the RNase-like effector BEC1054 (synonym CSEP0064). In order to identify host proteins targeted by BEC1054, recombinant BEC1054 was expressed in E. coli, solubilized, and used in pull-down assays from barley protein extracts. Many putative interactors were identified by LC-MS/MS after subtraction of unspecific binders in negative controls. Therefore, a directed yeast-2-hybrid assay, developed to measure the effectiveness of the interactions in yeast, was used to validate putative interactors. We conclude that BEC1054 may target several host proteins, including a glutathione-S-transferase, a malate dehydrogenase, and a pathogen-related-5 protein isoform, indicating a possible role for BEC1054 in compromising well-known key players of defense and response to pathogens. In addition, BEC1054 interacts with an elongation factor 1 gamma. This study already sugge...Continue Reading

References

Oct 7, 1991·FEBS Letters·J HejgaardI Svendsen
Nov 1, 1988·The Journal of Clinical Endocrinology and Metabolism·M I SurksL E Shapiro
Jan 1, 1987·Methods in Enzymology·J D BoekeG R Fink
Apr 2, 1999·European Journal of Biochemistry·S X Chen, P Schopfer
Aug 25, 1999·Current Opinion in Plant Biology·G P Bolwell
Dec 22, 1999·Trends in Ecology & Evolution·I FerriereI Krackow
Feb 16, 2002·Science·Cristina AzevedoPaul Schulze-Lefert
Mar 19, 2002·Genome Biology·David P DixonRobert Edwards
May 9, 2002·Journal of Experimental Botany·G Paul BolwellFarida Minibayeva
Jul 2, 2002·Plant Molecular Biology·Ulrich WagnerFelix Mauch
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Jan 2, 2003·Annals of Botany·Olga BlokhinaKurt V Fagerstedt
Dec 19, 2003·Biochemical and Biophysical Research Communications·Fang WuWeimin Gong
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Sep 1, 2005·Journal of Biomolecular Screening·Ronald I W OsmondAnthony R Dyer
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M LegrandB Fritig
Apr 11, 2006·Annual Review of Phytopathology·L C van LoonC M J Pieterse
Dec 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yulei ShangJian-Min Zhou
Mar 24, 2007·Molecular Plant-microbe Interactions : MPMI·Torben GjettingMichael F Lyngkjaer
Nov 19, 2008·Annual Review of Plant Biology·Ken Shirasu
Feb 25, 2009·Molecular Plant Pathology·Sandra NoirRalph Panstruga
Apr 3, 2009·Journal of Proteome Research·Markus BroschJyoti Choudhary
Jun 30, 2009·Proteomics·Dale GodfreyHans Thordal-Christensen
Jul 16, 2009·Molecular & Cellular Proteomics : MCP·Laurence V BindschedlerPietro D Spanu

❮ Previous
Next ❯

Citations

Feb 26, 2016·Frontiers in Plant Science·Laurence V BindschedlerPietro D Spanu
Apr 10, 2017·Plant Cell Reports·Irini Nianiou-ObeidatNikolaos E Labrou
Feb 15, 2018·Frontiers in Microbiology·David BoteroSilvia Restrepo
May 15, 2018·Journal of Experimental Botany·Mathias NottensteinerRalph Hückelhoven
Jun 28, 2018·Journal of Experimental Botany·Björn Sabelleck, Ralph Panstruga
Mar 8, 2018·Molecular Plant-microbe Interactions : MPMI·Jesús Martínez-CruzAlejandro Pérez-García
Mar 27, 2020·Frontiers in Plant Science·Luisa Katharina SchaeferSalim Bourras
Mar 3, 2020·Frontiers in Plant Science·Francisco J de Lamo, Frank L W Takken
Feb 26, 2021·The Plant Journal : for Cell and Molecular Biology·Beatrice ManserJavier Sánchez-Martín
Aug 13, 2021·Journal of Integrative Plant Biology·Muhammad TariqjaveedWenxian Sun
Sep 28, 2021·Frontiers in Plant Science·Hongbo YuanQian-Hua Shen
Oct 16, 2021·Molecular Plant-microbe Interactions : MPMI

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.