Interactions between thrombin and natural products of Millettia nitita var. hirsutissima using capillary zone electrophoresis

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Shuyu ZhangHong Liang

Abstract

A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between natural products isolated from Millettia nitita var. hirsutissima and thrombin qualitatively and quantitatively for the first time. The results showed that, compared with positive and negative control, the compounds ZYY-5 (genistein-8-C-beta-d-apiofuranosyl-(1-->6)-O-beta-d-glucopyranoside), ZYY-6 (calycosin), ZYY-8 (isoliquiritigenin), ZYY-9 (formononetin), ZYY-12 (gliricidin), ZYY-13 (8-O-methylretusin), FJ-2 (dihydrokaempferol), FJ-3 (biochanin), FJ-5 (afromosin) and XC-2 (hirsutissimiside F) interacted with thrombin, while ZYY-1 (sphaerobioside), ZYY-2 (formononetin-7-O-beta-d-apiofuranosyl-(1-->6)-O-beta-d-glucopyranoside), ZYY-3 (genistein-5-methylether-7-O-alpha-l-rhamnopyranosyl-(1-->6)-O-beta-d-glucopyranoside), ZYY-4 (retusin-7,8-O-beta-d-diglucopyranoside), ZYY-7 (symplocoside), ZYY-10 (ononin), ZYY-11 (genistin), ZYY-14 (afromosin-7-O-beta-d-glucopyranoside), ZYY-15 (lanceolarin), FJ-1 (liquiritigenin), FJ-4 (7,2-dihydroxy,4-methoxyisoflavan) and XC-1 (sphaerobioside) had no binding to thrombin. This indicated that the reported CZE method for the determinatio...Continue Reading

References

Jun 17, 1998·Forensic Science International : Synergy·P G Righetti, C Gelfi
Nov 3, 2007·Phytochemistry·Juan Carlos EspínFrancisco A Tomás-Barberán

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

Yao xue xue bao = Acta pharmaceutica Sinica
Cheng XiangJie Feng
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Jie FengYuying Zhao
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Jun ChengYuying Zhao
Chemical & Pharmaceutical Bulletin
Jun ChengHong Liang
© 2022 Meta ULC. All rights reserved