Interactions between Viral Regulatory Proteins Ensure an MOI-Independent Probability of Lysogeny during Infection by Bacteriophage P1.

MBio
Kailun ZhangLanying Zeng

Abstract

Phage P1 is a temperate phage which makes the lytic or lysogenic decision upon infecting bacteria. During the lytic cycle, progeny phages are produced and the cell lyses, and in the lysogenic cycle, P1 DNA exists as a low-copy-number plasmid and replicates autonomously. Previous studies at the bulk level showed that P1 lysogenization was independent of multiplicity of infection (MOI; the number of phages infecting a cell), whereas lysogenization probability of the paradigmatic phage λ increases with MOI. However, the mechanism underlying the P1 behavior is unclear. In this work, using a fluorescent reporter system, we demonstrated this P1 MOI-independent lysogenic response at the single-cell level. We further observed that the activity of the major repressor of lytic functions (C1) is a determining factor for the final cell fate. Specifically, the repression activity of P1, which arises from a combination of C1, the anti-repressor Coi, and the corepressor Lxc, remains constant for different MOI, which results in the MOI-independent lysogenic response. Additionally, by increasing the distance between phages that infect a single cell, we were able to engineer a λ-like, MOI-dependent lysogenization upon P1 infection. This suggests...Continue Reading

References

Mar 28, 1977·Molecular & General Genetics : MGG·P PrentkiL Caro
Feb 21, 1975·Science·D Kaiser, M Dworkin
Aug 1, 1975·Virology·H Greer
Jun 25, 1992·Nucleic Acids Research·M Citron, H Schuster
Oct 11, 1989·Nucleic Acids Research·F A OsborneB R Baumstark
Apr 12, 1973·Molecular & General Genetics : MGG·P Kourilsky
Sep 1, 1974·Journal of Bacteriology·F C NeidhardtD F Smith
Apr 13, 1968·Nature·M Okada, T Watanabe
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·H Ikeda, J Tomizawa
Oct 1, 1984·Journal of Bacteriology·R SandulacheD Kamp
Aug 1, 1995·FEMS Microbiology Reviews·J HeinrichH Schuster
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jul 17, 2003·Molecular Microbiology·Ivy F LauDavid J Sherratt
Oct 19, 2004·Journal of Bacteriology·Małgorzata B ŁobockaFrederick R Blattner
Jan 4, 2006·Journal of Biotechnology·Changsoo LeeSeokhwan Hwang
May 17, 2007·Molecular Microbiology·Tatyana RomantsovJanet M Wood
Mar 28, 2008·Molecular Microbiology·Rotem EdgarAmos B Oppenheim
Jun 22, 2011·Biophysical Journal·Eli RothenbergIdo Golding
Aug 20, 2014·Antimicrobial Agents and Chemotherapy·Typhaine Billard-PomaresCatherine Branger
Sep 12, 2014·Nucleic Acids Research·Jack A BryantDavid J Lee
Jan 20, 2015·Plasmid·Maryury Brown-JaqueMaite Muniesa
Apr 23, 2015·Biophysical Journal·Qiuyan ShaoLanying Zeng
Jul 21, 2015·Frontiers in Microbiology·Somenath BakshiJames C Weisshaar

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
smFISH

Software Mentioned

Schnitzcells
smFISH
TensorFlow
NIS
Elements
Spätzcells

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.