Interactions of a mammalian beta-galactoside-binding lectin with hamster fibroblasts

Journal of Cellular Biochemistry
D StojanovicR A Childs

Abstract

A beta-galactoside-binding endogenous lectin extracted from bovine heart binds to the surface of baby hamster kidney (BHK) cells. The binding to and agglutination of cells is reduced in certain ricin-resistant mutants (Ric cells) in parallel with the decreased number of binding sites for the selective agent, ricin, a galactose-specific plant lectin. However, clear differences in the binding specificities of bovine lectin and ricin are shown by the effect of neuraminidase. BHK cells and Ric mutant cells treated with neuraminidase bind similar amounts of the bovine lectin compared with untreated cells, and ricin binding is greatly increased. The mammalian lectin immobilised on inert glass mediates the attachment and spreading of normal BHK cells and agglutinates these cells in solution. Ricin-resistant mutant cells respond poorly. These results are consistent with a role of endogenous lectins in cellular adhesiveness and show that cell adhesion may be regulated by the density of specific surface receptors for lectins.

References

Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Jan 15, 1976·The Biochemical Journal·A MeagerR C Hughes
May 1, 1981·Analytical Biochemistry·J D Aplin, R C Hughes

❮ Previous
Next ❯

Citations

Oct 3, 2013·Plant Signaling & Behavior·A G Ingale, A U Hivrale
Mar 22, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Frédéric van den BrûleVincent Castronovo
Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·A RazR Lotan
Jan 1, 1984·Biochimica Et Biophysica Acta·E Roos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.