Interactions of anti-poly[d(G-br5C)] IgG with synthetic, viral and cellular Z DNAs

Journal of Biomolecular Structure & Dynamics
D A ZarlingT M Jovin

Abstract

Enzymatically synthesized poly[d(G-br5C)] was used to prepare specific polyclonal and monoclonal anti-Z DNA IgGs. The binding specificities of these antibodies were characterized using left-handed polynucleotides with the sequences d(G-x5C)n and d(A-x5C)n.d(G-T)n (mean = aza, methyl, bromo, or iodo). Polyclonal anti-poly[d(G-br5C)] IgG binds the convex surface of the Z helix as evidenced by the strong requirement for a methyl or halogen group at the C5 position of cytosine. Little or no anti-poly[d(G-br5C)] IgG binding occurs to left-handed DNAs carrying a phosphorothioate substitution in the dGpdC bond or an N-5 aza substitution in the cytosine ring. Anti-poly[d(G-br5C)] IgG can stabilize transient Z DNA structures in both polymer families, thereby displacing the equilibrium in solution between the right-and left-handed DNA conformations. Anti-poly[d(G-br5C)] IgG binding sites are found in all tested covalently closed circular natural DNAs (Form I) at their extracted negative superhelical densities, but not in any of the corresponding relaxed Form II or linear Form III DNAs. Binding of anti-poly[d(G-br5-C)] IgG leads to a reduction in the electrophoretic mobility of Form I DNA (e.g. SV40, phi X174, or pBR322) and to the format...Continue Reading

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·G M Rubin
Aug 1, 1976·Nucleic Acids Research·A J VarshavskyG P Georgiev
Aug 4, 1978·Science·U MüllerW Keller
Jan 1, 1978·Annual Review of Biophysics and Bioengineering·W R Bauer
Aug 1, 1977·Annals of the Rheumatic Diseases·I M HunneyballD R Stanworth
Oct 1, 1975·British Journal of Haematology·R H Dixon, W F Rosse
Jan 1, 1972·Annual Review of Biochemistry·M MeselsonJ Heywood
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·A RichF Azorin
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·T O'ConnorR D Wells
Sep 14, 1981·FEBS Letters·B Malfoy, M Leng
May 15, 1984·Journal of Molecular Biology·A UdvardyP Schedl
Feb 29, 1980·Science·N R Cozzarelli
Apr 15, 1980·Virology·E Fanning, I Baumgartner
Nov 7, 1980·Science·A Razin, A D Riggs
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M Behe, G Felsenfeld
Jan 1, 1981·Annual Review of Biochemistry·K Geider, H Hoffmann-Berling
Jan 1, 1981·Annual Review of Biochemistry·M Gellert
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C K SingletonR D Wells
Jun 11, 1983·Nucleic Acids Research·M W KilpatrickR D Wells
Jan 1, 1983·Annual Review of Biochemistry·W Doerfler
Mar 3, 1983·Nature·R M WalmsleyT D Petes
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·T M JovinM Robert-Nicoud
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J C WangK Becherer
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R ThomaeF M Pohl
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·T M JovinF Eckstein
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·D B Haniford, D E Pulleyblank
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J Arndt-JovinT M Jovin
Sep 1, 1984·Journal of Gerontology·P Hanley-Dunn, J L McIntosh
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·A Rich
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·F M Pohl
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J BallW T Garrard
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L J Peck, J C Wang
May 1, 1984·Archives of Internal Medicine·E J Zarling, L E Thompson
Jan 1, 1981·Annual Review of Genetics·A C Spradling, G M Rubin
Apr 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·A D AdoV I Dontsov
Jul 7, 1983·Nature·L P McIntoshT M Jovin
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J NickolG Felsenfeld
Dec 9, 1982·Nature·F M PohlE DiCapua

❮ Previous
Next ❯

Citations

Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A ZarlingA H Zarling
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·T M JovinF Eckstein
Nov 14, 1991·Biochemical and Biophysical Research Communications·J DelicM Moisan-Coppey
Dec 1, 1984·Journal of Biomolecular Structure & Dynamics·E M Lafer, B D Stollar
Oct 1, 1984·Bioscience Reports·F D MillerJ H van de Sande
Jan 1, 1986·CRC Critical Reviews in Biochemistry·B D Stollar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.