PMID: 6409611Aug 1, 1983Paper

Interactions of clathrin coat with model lipid membranes

European Journal of Biochemistry
E RalstonY Engelborghs

Abstract

The interaction of coat proteins from coated vesicles with model lipid membranes was examined using small unilamellar vesicles of dipalmitoylglycerophosphocholine as model membranes. Changes in membrane permeability were measured by the leakage of entrapped fluorescent dye, carboxyfluorescein. Both clathrin and the 55000-Da protein were found to be active. Density gradient centrifugation showed the formation of an irreversible protein-lipid complex. Dynamic light-scattering measurements showed that this complex is significantly larger than the original vesicles, suggesting that fusion is induced. The effects of pH, urea, Tris and ionic strength were studied and the possible biological relevance of the results is discussed.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M P Woodward, T F Roth
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W SchookS Kochwa
Jun 21, 1979·Nature·J L GoldsteinM S Brown
Sep 5, 1975·Journal of Molecular Biology·B M Pearse
May 1, 1981·The Journal of Cell Biology·J L RubensteinJ E Rothman
Jan 1, 1981·Annual Review of Biochemistry·B M Pearse, M S Bretscher
Mar 1, 1981·Cell·T Kirchhausen, S C Harrison
Dec 1, 1981·The Journal of Cell Biology·R A Crowther, B M Pearse
Jan 29, 1981·Nature·E Ungewickell, D Branton

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Citations

Feb 1, 1986·Chemistry and Physics of Lipids·S Massari, R Colonna
Dec 1, 1989·Alcoholism, Clinical and Experimental Research·A S WaliaE W Lamon
Jan 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·V J CunninghamA K Jones
Dec 15, 1986·Biochemical and Biophysical Research Communications·M Pallini, J Bramhall

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