Interactions of colipase with bile salt micelles. 1. Ultracentrifugation studies.

European Journal of Biochemistry
M CharlesP Desnuelle

Abstract

A detailed investigation by ultracentrifugation of the colipase-taurodeoxycholate system showed the formation of well-defined mixed associations with a sedimentation coefficient of about 2.2S. The fact that these associations were only detectable above the critical micelle concentration of the salt indicated that micelles rather than monomers were bound to the cofactor. Two technical difficulties must be overcome before the weight of the associations could be measured with a reasonable accuracy. Firstly, the partial specific volume of the associations was determined using a digital microdensimeter and the interferometric system of the ultracentrifuge for concentration determinations. Secondly, due to the fact that micelle concentrations could not be equilibrated by dialysis, even after an extended period of time, an appropriate dilution of the ligand in the buffer compartment was necessary in order to compensate for its fixation by colipase in the solution. Then, the ionic strength dependence of the weight of the associations was found to vary in parallel with that of the micelles and to be in each case equal to the sum of the weights of one colipase molecule and one micelle. Therefore, colipase can be expected to contain a sin...Continue Reading

References

Jul 7, 1974·Biochimica Et Biophysica Acta·C ErlansonP Desnuelle
May 1, 1973·Biochemical and Biophysical Research Communications·M F MayliéP Desnuelle
Jun 15, 1973·Biochimica Et Biophysica Acta·C ErlansonB Borgström
Jul 7, 1974·Biochimica Et Biophysica Acta·M CharlesM Rovery
Oct 5, 1971·Biochimica Et Biophysica Acta·R G Morgan, N E Hoffman
Jan 19, 1971·Biochimica Et Biophysica Acta·M F MayliéP Desnuelle
Mar 1, 1964·Biochemistry·D A YPHANTIS

❮ Previous
Next ❯

Citations

Jan 1, 1977·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C LegerJ Flanzy
Oct 15, 1975·European Journal of Biochemistry·H SariP Desnuelle
Sep 15, 1976·European Journal of Biochemistry·S BonJ Massoulié
Dec 1, 1976·European Journal of Biochemistry·V Fernandez-LopezG Ailhaud
Apr 1, 1982·European Journal of Biochemistry·M PierrotJ Drenth
Feb 1, 1982·The Journal of Clinical Investigation·K J GaskinG G Forstner
Nov 6, 1989·FEBS Letters·C ChaillanD Lombardo
Apr 28, 1978·Biochimica Et Biophysica Acta·J S PattonB Borgström
Mar 21, 1980·Biochimica Et Biophysica Acta·C Erlanson-Albertsson
Dec 20, 1976·Biochimica Et Biophysica Acta·B Borgström, J Donnér
Sep 1, 1988·Biochimie·C ChapusR Verger
Oct 28, 1981·Biochimica Et Biophysica Acta·P CanioniL Sarda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.