Interactions of colipase with bile salt micelles. 2. Study by dialysis and spectrophotometry.

European Journal of Biochemistry
H SariP Desnuelle

Abstract

The finding reported in the preceding paper that colipase is able to bind one sodium taurodeoxycholate micelle per molecule was confirmed by dialysis and spectrophotometry. Dialysis in the presence of labelled sodium taurodeoxycholate provided a direct qualitative proof of taurodeoxycholate binding to colipase. This binding was found to occur only above the critical micelle concentration. But, dialysis did not give any information about the composition of the associations, because equilibrium was not attained at the end of the assays. Addition of sodium taurodeoxycholate above the critical micelle concentration was also observed to induce a strong perturbation of the ultraviolet spectrum of one or several of the three tyrosines of colipase. The variation of the perturbation as a function of sodium taurodeoxycholate concentration was consistent with the binding of a single micelle to colipase. The dissociation constant calculated in "micelle molarity" was approximately 1 X 10(-4) M. The colipase-bile salt micelle association can fix one molecule of lipase to form a ternary complex which represents an interesting model of a protein-protein interaction mediated by an organized lipid structure. The ternary complex is probably also ...Continue Reading

References

Oct 15, 1975·European Journal of Biochemistry·M CharlesP Desnuelle
Apr 1, 1973·Chemistry and Physics of Lipids·H Brockerhoff
May 1, 1973·Biochemical and Biophysical Research Communications·M F MayliéP Desnuelle
Aug 1, 1973·European Journal of Biochemistry·B Borgström, C Erlanson
Jul 7, 1974·Biochimica Et Biophysica Acta·M CharlesM Rovery
Jun 4, 1968·Biochimica Et Biophysica Acta·B Entressangles, P Desnuelle

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Citations

Jan 1, 1977·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C LegerJ Flanzy
Dec 1, 1976·European Journal of Biochemistry·V Fernandez-LopezG Ailhaud
Apr 1, 1982·European Journal of Biochemistry·M PierrotJ Drenth
Nov 6, 1989·FEBS Letters·C ChaillanD Lombardo
Nov 1, 1991·International Journal of Peptide and Protein Research·B BellonL Sarda
Jun 19, 1998·Protein Expression and Purification·R A Cordle, M E Lowe
Jan 18, 1984·Biochimica Et Biophysica Acta·B SternbyB Borgström
Oct 24, 1977·Biochimica Et Biophysica Acta·C ErlansonB Borgström
Dec 29, 1981·Biochimica Et Biophysica Acta·J de CaroM Rovery
Apr 28, 1978·Biochimica Et Biophysica Acta·J S PattonB Borgström
Mar 21, 1980·Biochimica Et Biophysica Acta·C Erlanson-Albertsson
Apr 8, 1992·Biochimica Et Biophysica Acta·C Erlanson-Albertsson
Sep 1, 1988·Biochimie·C ChapusR Verger
Oct 5, 1989·Biochimica Et Biophysica Acta·L de La FournièreL Sarda
Dec 30, 1992·Biochemical and Biophysical Research Communications·N RuganiJ Rathelot

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