Interactions of cytoplasmic dynein light chains Tctex-1 and LC8 with the intermediate chain IC74

Biochemistry
Moses MakokhaElisar Barbar

Abstract

The interactions of three subunits of cytoplasmic dynein from Drosophila melanogaster, LC8, Tctex-1, and the N-terminal domain of IC74 (N-IC74, residues 1-289), were characterized in vitro by affinity methods, limited proteolysis, and circular dichroism spectroscopy. These subunits were chosen for study because they are presumed to promote the assembly of the complex and to be engaged in the controlled binding and release of cargo. Limited proteolysis and mass spectrometry of N-IC74 in the presence of LC8 and Tctex-1 localized binding of Tctex-1 to the vicinity of K104 and K105, and localized binding of LC8 to the region downstream of K130. Circular dichroism, fluorescence, sedimentation velocity, and proteolysis studies indicate that N-IC74 has limited secondary and tertiary structure at near physiological solution conditions. Upon addition of LC8, N-IC74 undergoes a significant conformational change from largely unfolded to a more ordered structure. This conformational change is reflected in increased global protection of N-IC74 from proteolytic digestion following the interaction, and in a significant change in the CD signal. A smaller but reproducible change in the CD spectra was observed upon Tctex-1 binding as well. The i...Continue Reading

References

Dec 1, 1995·The Journal of Biological Chemistry·S Karki, E L Holzbaur
Jun 1, 1994·Molecular Biology of the Cell·S R GillT A Schroer
Feb 11, 1999·The Journal of Cell Biology·G J PazourG B Witman
Feb 27, 1999·Current Opinion in Cell Biology·S Karki, E L Holzbaur
Nov 5, 1999·Journal of Molecular Biology·P E Wright, H J Dyson
Mar 21, 2000·Biochimica Et Biophysica Acta·S J SusalkaK K Pfister

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Citations

Jun 1, 2006·Biochemistry·Matthew TalbottElisar Barbar
Sep 18, 2009·The Journal of Biological Chemistry·Justin HallElisar Barbar
Oct 5, 2010·The Journal of Biological Chemistry·László RadnaiLászló Nyitray
Oct 27, 2010·The Journal of Biological Chemistry·Afua Nyarko, Elisar Barbar
Sep 21, 2011·The Journal of Biological Chemistry·Jessica L MorganElisar Barbar
Jun 7, 2012·The Journal of Biological Chemistry·Afua NyarkoElisar Barbar
Oct 21, 2003·Molecular Biology of the Cell·Stephen J KingTrina A Schroer
Mar 17, 2004·Molecular Biology of the Cell·Anindya Ghosh-RoyKrishanu Ray
Oct 8, 2004·Molecular Biology of the Cell·Triscia W HendricksonWinfield S Sale
Jun 3, 2011·Molecular Biology of the Cell·Melissa D Stuchell-BreretonJohn A Cooper
Jan 26, 2011·Molecular and Cellular Biology·Nathan J PavlosJiake Xu
Jan 28, 2006·PLoS Genetics·K Kevin PfisterElizabeth M C Fisher
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tianhua ZhouRaymond L Erikson
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gene S TanMatthias J Schnell
Oct 1, 2013·Applied Microbiology and Biotechnology·M A S ToledoA R Azzoni
Oct 25, 2014·Nature Communications·Mingyue JinShinji Hirotsune
Aug 7, 2013·Biochemistry·Ariam I KidaneElisar Barbar
May 31, 2011·Biophysical Chemistry·Afua NyarkoElisar Barbar
May 14, 2005·EMBO Reports·Suresh K RayalaRakesh Kumar
Jan 6, 2004·Journal of Neurobiology·Richard B ValleeLora E Barnhart
Nov 10, 2007·Reviews in Medical Virology·Russell J DiefenbachAnthony L Cunningham
Dec 21, 2010·Cellular Microbiology·Marc A SchneiderCarsten Lambert
Jul 23, 2011·The FEBS Journal·Javier Merino-GraciaIgnacio Rodríguez-Crespo
Jul 23, 2011·The FEBS Journal·Péter RapaliLászló Nyitray
Nov 21, 2009·The EMBO Journal·Anat ShmueliOrly Reiner
Jan 29, 2014·Journal of Basic Microbiology·Jie LiuZhensheng Kang
Apr 20, 2004·Current Opinion in Structural Biology·Stan A Burgess, Peter J Knight
Oct 25, 2003·Biochemical and Biophysical Research Communications·Maki SugaiJun Sukegawa
Apr 24, 2014·Computational and Structural Biotechnology Journal·Elisar Barbar, Afua Nyarko
Oct 23, 2012·Spermatogenesis·Richard Zoller, Cordula Schulz
Mar 18, 2009·Methods in Enzymology·Gregory Benison, Elisar Barbar
Jul 28, 2004·Journal of Molecular and Cellular Cardiology·Songhai ChenHeidi E Hamm
Feb 11, 2005·The Journal of Biological Chemistry·John C WilliamsWayne A Hendrickson

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