Interactions of endoglucanases with amorphous cellulose films resolved by neutron reflectometry and quartz crystal microbalance with dissipation monitoring

Langmuir : the ACS Journal of Surfaces and Colloids
Gang ChengMichael S Kent

Abstract

A study of the interaction of four endoglucanases with amorphous cellulose films by neutron reflectometry (NR) and quartz crystal microbalance with dissipation monitoring (QCM-D) is reported. The endoglucanases include a mesophilic fungal endoglucanase (Cel45A from H. insolens), a processive endoglucanase from a marine bacterium (Cel5H from S. degradans ), and two from thermophilic bacteria (Cel9A from A. acidocaldarius and Cel5A from T. maritima ). The use of amorphous cellulose is motivated by the promise of ionic liquid pretreatment as a second generation technology that disrupts the native crystalline structure of cellulose. The endoglucanases displayed highly diverse behavior. Cel45A and Cel5H, which possess carbohydrate-binding modules (CBMs), penetrated and digested within the bulk of the films to a far greater extent than Cel9A and Cel5A, which lack CBMs. While both Cel45A and Cel5H were active within the bulk of the films, striking differences were observed. With Cel45A, substantial film expansion and interfacial broadening were observed, whereas for Cel5H the film thickness decreased with little interfacial broadening. These results are consistent with Cel45A digesting within the interior of cellulose chains as a clas...Continue Reading

References

Jun 1, 1995·Protein Science : a Publication of the Protein Society·M LinderA Annila
Jan 1, 1997·Biotechnology Advances·M K Bhat, S Bhat
Mar 9, 2005·Journal of Colloid and Interface Science·Jonny ErikssonKatja S Johansen
May 11, 2006·Biotechnology and Bioengineering·Y-H Percival Zhang, Lee R Lynd
May 13, 2006·Biotechnology Advances·Y-H Percival ZhangJonathan R Mielenz
Mar 8, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Xavier TuronRandall S Deinhammer
May 31, 2008·Biotechnology and Bioengineering·Sanni P VoutilainenAnu Koivula
Apr 8, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Christian AulinLars Wågberg
Jul 21, 2009·Journal of Bacteriology·Brian J WatsonSteven W Hutcheson
Jul 23, 2009·Acta Crystallographica. Section D, Biological Crystallography·Jose Henrique PereiraPaul D Adams
Aug 29, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Frank HeinrichMathias Lösche
Jan 7, 2010·The Journal of Physical Chemistry. B·Gregg T BeckhamMichael F Crowley
May 28, 2010·Biotechnology and Bioengineering·Seth E LevineDouglas S Clark
Aug 14, 2010·Science·Chris SomervilleStephen P Long
Feb 8, 2011·Biomacromolecules·Eero KontturiRitva Serimaa

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Citations

Jul 4, 2013·Biotechnology for Biofuels·Vimalier Reyes-OrtizDanielle Tullman-Ercek
Jan 26, 2013·Journal of Colloid and Interface Science·S A MaurerC J Radke
Jun 19, 2015·Applied Microbiology and Biotechnology·Chao WangKai Zhang
Feb 11, 2014·Carbohydrate Polymers·Chao Wang, Alan R Esker
Nov 28, 2020·Biotechnology and Bioengineering·Bhargava NemmaruShishir P S Chundawat
Apr 28, 2021·International Journal of Biological Macromolecules·Madhu Nair MuraleedharanAji P Mathew
Mar 5, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Feng JiangMaren Roman
Apr 4, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Mei CuiRongxin Su

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