PMID: 8955414Dec 1, 1996Paper

Interactions of Escherichia coli UmuD with activated RecA analyzed by cross-linking UmuD monocysteine derivatives

Journal of Bacteriology
M H Lee, G C Walker

Abstract

SOS mutagenesis in Escherichia coli requires the participation of a specialized system involving the activated form of UmuD (UmuD'), UmuC, RecA, and DNA polymerase III proteins. We have used a set of monocysteine derivatives of UmuD (M. H. Lee, T. Ohta, and G. C. Walker, J. Bacteriol. 176:4825-4837, 1994) and the cysteine-specific photoactive cross-linker p-azidoiodoacetanilide (AIA) to study not only the interactions of intact UmuD in the homodimer but also the interactions of UmuD with activated RecA. The reactivities of the individual UmuD monocysteine derivatives with AIA were similar to their reactivities with iodoacetate. The relative efficiencies of cross-linking of the AIA-modified monocysteine UmuD derivatives in the homodimer form are also consistent with our previous conclusions concerning the relative closeness of various UmuD residues to the dimer interface. With respect to the UmuD-RecA interface, the AIA-modified VC34 and SC81 monocysteine derivatives cross-linked most efficiently with RecA, indicating that positions 34 and 81 of UmuD are closer to the RecA interface than the other positions we tested. The AIA-modified SC57, SC67, and SC112 monocysteine derivatives cross-linked moderately efficiently with RecA. N...Continue Reading

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Citations

Nov 13, 2002·The Journal of Biological Chemistry·Elizabeth A StohlH Steven Seifert
May 8, 2019·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Petr GrúzTakehiko Nohmi
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·T OppermanG C Walker
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·S M SimonG C Walker
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mark D SuttonGraham C Walker
Oct 12, 2010·Journal of Nucleic Acids·Jaylene N OllivierrePenny J Beuning
Nov 28, 2000·Annual Review of Genetics·M D SuttonG C Walker
May 9, 2001·Mutation Research·G C Walker
Dec 1, 1996·Journal of Bacteriology·M H LeeG C Walker
Dec 27, 2007·Molecular Cell·Veronica G GodoyGraham C Walker

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