PMID: 8939452Oct 1, 1996Paper

Interactions of glycine and strychnine with their receptor recognition sites in mouse spinal cord

Neurochemistry International
V O'ConnorJ P Fry

Abstract

Interactions between the inhibitory neurotransmitter glycine and its receptor antagonist strychnine have been studied in mouse spinal cord membranes and both agents employed to protect against residue selective protein modifying reagents in order to identify contact residues for ligand binding. Glycine was found to behave as a full competitive inhibitor of [3H]-strychnine binding, provided that precautions were taken to prevent radioligand binding to the glass-fibre filters used to terminate the assays. Hill coefficients for the glycine inhibition of [3H]-strychnine binding were not significantly different from one, indicating a lack of cooperative interactions. For the protection experiments, N-bromosuccinimide, tetranitromethane, diethylpyrocarbonate and 2,3-butanedione were used under conditions selective for tryptophan, tyrosine, histidine and arginine residues, respectively. Of these reagents, N-bromosuccinimide, tetranitromethane and diethylpyrocarbonate caused a decrease in total [3H]-strychnine binding without affecting the ability of unlabelled strychnine to compete. In contrast, the same reagents disrupted the ability of glycine to inhibit [3H]-strychnine binding. The presence of either excess glycine (10(-2) M) or st...Continue Reading

References

Jul 31, 1979·Molecular and Cellular Biochemistry·J F Riordan
Jun 1, 1978·Journal of Neurochemistry·D LeFortM Cuénod
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R J VandenbergP R Schofield
Sep 1, 1992·Neuron·R J VandenbergP R Schofield
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D LangoschH Betz
May 1, 1989·Journal of Neurochemistry·P P PhelanG A Johnston
Jun 1, 1989·British Journal of Pharmacology·N TokutomiN Akaike
Nov 1, 1985·Journal of Pharmacological Methods·G A McPherson
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A B Young, S H Snyder
Jun 29, 1971·Experimental Brain Research·D R CurtisG A Johnston
Apr 24, 1984·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C B GundersenI Parker
Apr 5, 1983·European Journal of Biochemistry·D GrahamH Betz
Apr 1, 1981·Journal of Neurochemistry·J M Schaeffer, S M Anderson
Mar 1, 1949·Journal of the American Chemical Society·G FODOR, O KOVACS

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Citations

Jun 24, 2004·Neurochemistry International·Tímea Bíró, Gábor Maksay
Aug 20, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Karim RaafatHans-Georg Breitinger
Jan 18, 2005·Alcoholism, Clinical and Experimental Research·Anna Molander, Bo Söderpalm
Dec 31, 2002·Neuropharmacology·Gábor Maksay, Tímea Bíró
May 12, 2020·ACS Chemical Neuroscience·Ulrike Breitinger, Hans-Georg Breitinger

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