Interactions of hemoglobin and myoglobin with their ligands CN(-), CO, and O2 monitored by electrospray ionization-mass spectrometry

Analytical Chemistry
Modupeola A SowoleLars Konermann

Abstract

Electrospray ionization (ESI)-mass spectrometry (MS) can provide information on protein-ligand interactions via detection of the corresponding complexes as gaseous ions. Unfortunately, some systems are prone to dissociation upon transfer into the gas phase. The reversible oxygen binding to hemoglobin (Hb) has been extensively studied in solution using a wide range of biophysical techniques. In addition to O2, ferrous (Fe(II)) Hb can bind CO. High affinity interactions with CN(-) are limited to the ferric (Fe(III)) state. In analogous fashion, CN(-), CO, and O2 bind to myoglobin (Mb). It remains unclear whether any of these ligand-bound forms can be observed by ESI-MS. In this work we demonstrate the successful detection of MbCN, while MbCO and MbO2 do not survive under ESI-MS conditions. Control experiments suggest that an older report of "MbO2" detection by ESI-MS may involve the misassignment of oxidation artifacts formed under corona discharge conditions. The situation is more favorable for ESI-MS studies on Hb. The most intense signal in the HbCN mass distribution corresponds to the expected complex with four cyanide moieties bound. Ligand loss during ESI-MS is around 20%. HbCO is detectable as well, albeit with a more noti...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C A Reed, S K Cheung
Jan 3, 1992·Science·G K AckersM A Daugherty
Oct 5, 1980·Journal of Molecular Biology·S E Phillips
Aug 16, 1994·Biochemistry·Y Konishi, R Feng
Jan 1, 1996·Analytical Chemistry·M Wilm, M Mann
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·M F PerutzG G Dodson
Nov 26, 1998·The New England Journal of Medicine·A Ernst, J D Zibrak
Apr 14, 1999·Nature Structural Biology·W A EatonA Mozzarelli
Sep 28, 1999·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·E Sigfridsson, U Ryde
Jul 11, 2001·Journal of the American Society for Mass Spectrometry·J M WellsS A McLuckey
Oct 19, 2001·Journal of the American Society for Mass Spectrometry·A Schmidt, M Karas
May 9, 2002·Mass Spectrometry Reviews·S D Maleknia, K Downard
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joan J EnglanderVirgil L Woods
Jul 3, 2003·Mass Spectrometry Reviews·Julia Laskin, Jean H Futrell
Aug 27, 2003·Biochemistry·Victoria L RobinsonArthur Arnone
Sep 2, 2003·Angewandte Chemie·John B Fenn
Dec 31, 2003·Journal of the American Society for Mass Spectrometry·Brian N Green, Serge N Vinogradov
Mar 11, 2004·Chemical Reviews·Jonathan A Lukin, Chien Ho
Mar 17, 2004·Analytical Chemistry·Igor V Chernushevich, Bruce A Thomson
Dec 7, 2005·Rapid Communications in Mass Spectrometry : RCM·Kevin J Mark, D J Douglas
May 1, 1997·Journal of the American Society for Mass Spectrometry·D S GrossE R Williams
Jan 26, 2007·Analytical Chemistry·Maolian Chen, Kelsey D Cook
Mar 24, 2007·Analytical Chemistry·Peter NemesAkos Vertes
Apr 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alessandro ArcovitoStefano Della Longa
Jul 20, 2007·Physical Chemistry Chemical Physics : PCCP·Nick C Polfer, Jos Oomens
Jul 26, 2007·Chemical Reviews·Justin L P BeneschCarol V Robinson
Aug 19, 2007·Analytical Chemistry·Gary J Van Berkel, Vilmos Kertesz
Dec 27, 2008·Journal of the American Society for Mass Spectrometry·Charlotte A ScarffJames H Scrivens
Jun 25, 2009·Mass Spectrometry Reviews·Paul Kebarle, Udo H Verkerk
Nov 26, 2009·Journal of the American Society for Mass Spectrometry·Claudia BichRenato Zenobi
Feb 2, 2010·Journal of the American Society for Mass Spectrometry·Michal Sharon
Oct 23, 2010·Journal of the American Chemical Society·Francis O TalbotRebecca A Jockusch
Mar 15, 2011·The Journal of Physical Chemistry. B·Christopher J HoganJuan Fernandez de la Mora
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Yang KangD J Douglas
May 11, 2011·Angewandte Chemie·Natalia S NagornovaOleg V Boyarkin
May 14, 2011·Biochimica Et Biophysica Acta·Andrea Bellelli, Maurizio Brunori
Sep 2, 2011·Current Opinion in Structural Biology·Justin L P Benesch, Brandon T Ruotolo
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Atim A EnyenihiRoman A Zubarev
Jan 25, 2012·Journal of the American Society for Mass Spectrometry·Elena N KitovaJohn S Klassen

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Citations

Jan 21, 2018·Scientific Reports·Toshiki NagakuboMichihiko Kobayashi
Jan 8, 2020·Nanoscale·Pir MuhammadBingyang Shi
Mar 17, 2017·Annual Review of Analytical Chemistry·Joseph D EschweilerBrandon T Ruotolo

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