PMID: 1195333Apr 23, 1975Paper

Interactions of liver encoplasmic reticulum membranes and polysomes in vitro

The Journal of Membrane Biology
L SchiaffonatiA Bernelli-Zazzera

Abstract

The interactions of various preparations of endoplasmic reticulum membranes and polysomes have been studied by means of a sandwich sucrose gradient that clearly isolates free ribosomes, smooth endoplasmic reticulum (S.E.R.) and rough endoplasmic reticulum (R.E.R.) from the microsomal fraction of rat liver homogenates. Reconstructed rough membranes separate well from the native R.E.R. but occupy the same position along the gradient as the S.E.R. and the rough membranes, stripped of their ribosomes by means of LiCl. Native R.E.R. and S.E.R. do not bind any added labeled polysomes at 0 degree C; previous treatment with LiCl does not modify the behavior of S.E.R. The presence of cell sap during the binding reaction does not increase polysome fixation by stripped-rough membranes but protects in some way the polysomes and preserves all their original functional capacity of amino acid incorporation into protein.

References

Dec 1, 1969·The Biochemical Journal·T Scott-Burden, A O Hawtrey
Sep 18, 1972·European Journal of Biochemistry·C A BlythB R Rabin
Sep 1, 1972·The Biochemical Journal·F S Rolleston
Oct 15, 1973·Biochemical and Biophysical Research Communications·T Scott-Burden, A O Hawtray
Aug 1, 1974·Experimental and Molecular Pathology·F CajoneA Bernelli-Zazzera
Nov 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·T Scott-Burden, A O Hawtrey
Apr 1, 1973·The Biochemical Journal·F S Rolleston, D Mak
Sep 5, 1973·Biochemical and Biophysical Research Communications·T EkrenH C Pitot
Oct 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Dallner, L Ernster
Dec 3, 1971·Biochemical and Biophysical Research Communications·T K ShiresH C Pitot
Dec 1, 1970·Experimental and Molecular Pathology·G RagnottiA Bernelli-Zazzera

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Citations

Oct 26, 1976·Biochimica Et Biophysica Acta·P R McIntosh, K O'Toole
Apr 1, 1978·Experimental and Molecular Pathology·M E FerreroA Bernelli-Zazzera

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