Interactions of mRNAs and gRNAs involved in trypanosome mitochondrial RNA editing: structure probing of a gRNA bound to its cognate mRNA.

RNA
Laura E Yu, D J Koslowsky

Abstract

Expression of mitochondrial genes in Trypanosoma brucei requires RNA editing of its mRNA transcripts. During editing, uridylates are precisely inserted and deleted as directed by the gRNA template to create the protein open reading frame. This process involves the bimolecular interaction of the gRNA with its cognate pre-edited mRNA and the assembly of a protein complex with the enzymatic machinery required. While a considerable amount of work has been done identifying the protein components of the editing complex, very little is known about how a functional editosome is assembled. In addition, the importance of RNA structure in establishing a functional editing complex is poorly understood. Work in our lab suggests that different mRNA/gRNA pairs can form similar secondary structures suggesting that a common core architecture may be important for editosome recognition and function. Using solution structure probing, we have investigated the structure of the initiating gRNA, gCYb-558, in the mRNA/gRNA complex with pre-edited apocytochrome b mRNA. Our data indicate that the stem-loop formed by the guiding region of the gRNA alone is maintained in its interaction with the pre-edited message. In addition, our data suggest that a gRNA...Continue Reading

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Citations

Sep 22, 2012·Annual Review of Microbiology·H Ulrich Göringer
Nov 22, 2015·Bioinformatics·Laura DiChiacchioDavid H Mathews
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·H Ulrich GöringerCordula Böhm
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May 24, 2017·Nucleic Acids Research·Rachel M SimpsonLaurie K Read
Dec 13, 2006·The Journal of Biological Chemistry·Catherine Cifuentes-RojasJorge Cruz-Reyes

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