Interactions of N-stearoyl sphingomyelin with cholesterol and dipalmitoylphosphatidylcholine in bilayer membranes

Biophysical Journal
P R Maulik, G G Shipley

Abstract

Differential scanning calorimetry and x-ray diffraction have been utilized to investigate the interaction of N-stearoylsphingomyelin (C18:0-SM) with cholesterol and dipalmitoylphosphatidylcholine (DPPC). Fully hydrated C18:0-SM forms bilayers that undergo a chain-melting (gel -->liquid-crystalline) transition at 45 degrees C, delta H = 6.7 kcal/mol. Addition of cholesterol results in a progressive decrease in the enthalpy of the transition at 45 degrees C and the appearance of a broad transition centered at 46.3 degrees C; this latter transition progressively broadens and is not detectable at cholesterol contents of >40 mol%. X-ray diffraction and electron density profiles indicate that bilayers of C18:0-SM/cholesterol (50 mol%) are essentially identical at 22 degrees C and 58 degrees C in terms of bilayer periodicity (d = 63-64 A), bilayer thickness (d rho-p = 46-47 A), and lateral molecular packing (wide-angle reflection, 1/4.8 A-(1)). These data show that cholesterol inserts into C18:0-SM bilayers, progressively removing the chain-melting transition and altering the bilayer structural characteristics. In contrast, DPPC has relatively minor effects on the structure and thermotropic properties of C18:0-SM. DPPC and C18:0-SM ex...Continue Reading

Citations

Jun 6, 2002·Chemistry and Physics of Lipids·Rumiana Koynova, Martin Caffrey
Aug 7, 2002·Current Opinion in Structural Biology·Erwin London
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·H SprongG van Meer
Jul 22, 2010·The Journal of Biological Chemistry·Christian KloseKai Simons
Mar 23, 2002·The Journal of Biological Chemistry·Peter MattjusRhoderick E Brown
May 5, 2012·Progress in Lipid Research·Jean-Luc CacasSébastien Mongrand
Dec 29, 2009·FEBS Letters·Ana J García-Sáez, Petra Schwille
Oct 9, 2013·Biochimica Et Biophysica Acta·Itziar M D PosadaAlicia Alonso
Jan 5, 2011·Biochimica Et Biophysica Acta·Christian SergeliusShigeo Katsumura
Feb 11, 2014·Colloids and Surfaces. B, Biointerfaces·Aurora Dols-PerezGabriel Gomila
Mar 28, 2006·Biophysical Journal·Salvatore ChiantiaPetra Schwille
Dec 26, 2003·Biophysical Journal·Reiko IshitsukaToshihide Kobayashi
Feb 12, 2008·Biochimica Et Biophysica Acta·Alexander I GreenwoodStephanie Tristram-Nagle
Jul 24, 1998·Biochimica Et Biophysica Acta·V S Kulkarni, R E Brown
Oct 19, 2007·Biochimica Et Biophysica Acta·Alison L Costello, Todd M Alam
Oct 3, 2009·Biochimica Et Biophysica Acta·Marie-Cécile GiocondiChristian Le Grimellec
Dec 8, 2004·Biochimica Et Biophysica Acta·Bohdana TérováThomas K M Nyholm
Mar 1, 2003·Biophysical Journal·Jared C LawrenceRobert M Henderson
May 23, 2002·Biophysical Journal·Madalina RujoiM Cecilia Yappert
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Linda S HirstRobin L B Selinger
Jun 6, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Tomas Byström, Göran Lindblom
Mar 15, 2006·Biophysical Journal·Elizabeth J WallacePeter D Olmsted
Oct 25, 1996·The Journal of Biological Chemistry·M B Ruiz-ArgüelloA Alonso
Oct 16, 2015·Physical Chemistry Chemical Physics : PCCP·Kiyoshi YagiYuji Sugita
Nov 4, 2017·Physical Chemistry Chemical Physics : PCCP·A G Dos SantosL C Silva
Oct 30, 2010·Magnetic Resonance in Chemistry : MRC·A V FilippovB V Munavirov
Oct 13, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·F A M Leermakers, A L Rabinovich
Nov 22, 2001·The Journal of Biological Chemistry·Pierre Emmanuel MilhietChristian Le Grimellec
Sep 13, 2007·The Journal of Biological Chemistry·Ana J García-SáezPetra Schwille
Sep 19, 2019·Organic & Biomolecular Chemistry·Shinya HanashimaMichio Murata

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