Interactions of neurons with topographic nano cues affect branching morphology mimicking neuron-neuron interactions.

Journal of Molecular Histology
Koby BaranesOrit Shefi

Abstract

We study the effect of topographic nano-cues on neuronal growth-morphology using invertebrate neurons in culture. We use photolithography to fabricate substrates with repeatable line-pattern ridges of nano-scale heights of 10-150 nm. We plate leech neurons atop the patterned-substrates and compare their growth pattern to neurons plated atop non-patterned substrates. The model system allows us the analysis of single neurite-single ridge interactions. The use of high resolution electron microscopy reveals small filopodia processes that attach to the line-pattern ridges. These fine processes, that cannot be detected in light microscopy, add anchoring sites onto the side of the ridges, thus additional physical support. These interactions of the neuronal process dominantly affect the neuronal growth direction. We analyze the response of the entire neuronal branching tree to the patterned substrates and find significant effect on the growth patterns compared to non-patterned substrates. Moreover, interactions with the nano-cues trigger a growth strategy similarly to interactions with other neuronal cells, as reflected in their morphometric parameters. The number of branches and the number of neurites originating from the soma decreas...Continue Reading

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Citations

May 30, 2014·International Journal of Nanomedicine·Noa AlonOrit Shefi
Feb 21, 2014·Nanomedicine·Maha Elsayed, Olivia M Merkel
Mar 5, 2016·Nano Letters·Merav Antman-Passig, Orit Shefi
Aug 1, 2016·Biochimica Et Biophysica Acta·Luana HeimfarthRegina Pessoa-Pureur
Apr 25, 2017·Tissue Engineering. Part a·Merav Antman-PassigOrit Shefi
Jun 23, 2019·Developmental Neurobiology·Itai HennYossi Mandel
Feb 11, 2018·Journal of Biomedical Materials Research. Part a·Andreea BeluDirk Mayer
Dec 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ifat NissanAharon Gedanken
Jun 24, 2017·Advanced Healthcare Materials·Michal MarcusOrit Shefi
Apr 19, 2020·Developmental Neurobiology·Jeongyeon SeoInsung S Choi
Mar 20, 2020·Scientific Reports·Sharon CohenOrit Shefi
Nov 14, 2020·Advanced Healthcare Materials·Sung-Min KangJoong Yull Park
Sep 27, 2018·ACS Applied Materials & Interfaces·Olivia V CangellarisMartha U Gillette
Jul 2, 2021·Advanced Healthcare Materials·Sitara VedaramanLaura De Laporte

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