Interactions of Pseudomonas aeruginosa and Corynebacterium spp. with non-phagocytic brain microvascular endothelial cells and phagocytic Acanthamoeba castellanii

Parasitology Research
Ruqaiyyah SiddiquiNaveed Ahmed Khan

Abstract

Several lines of evidence suggest that Acanthamoeba interact with bacteria, which may aid in pathogenic bacterial transmission to susceptible hosts, and these interactions may have influenced evolution of bacterial pathogenicity. In this study, we tested if Gram-negative Pseudomonas aeruginosa and Gram-positive Corynebacterium spp. can associate/invade and survive inside Acanthamoeba castellanii trophozoites and cysts, as well as non-phagocytic human brain microvascular endothelial cells. The results revealed that both Corynebacterium spp. and P. aeruginosa were able to associate as well as invade and/or taken up by the phagocytic A. castellanii trophozoite. In contrast, P. aeruginosa exhibited higher association as well as invasion of non-phagocytic HBMEC compared with Corynebacterium spp. Notably, P. aeruginosa remained viable during the encystment process and exhibited higher levels of recovery from mature cysts (74.54 bacteria per amoebae) compared with Corynebacterium spp. (2.69 bacteria per amoeba) (P < 0.05). As Acanthamoeba cysts can be airborne, these findings suggest that Acanthamoeba is a potential vector in the transmission of P. aeruginosa to susceptible hosts. When bacterial-ridden amoebae were exposed to favourab...Continue Reading

References

Jan 1, 1991·Applied and Environmental Microbiology·C Paszko-KolvaR R Colwell
Apr 1, 1997·Current Microbiology·X Wang, D G Ahearn
Oct 29, 2000·International Journal of Antimicrobial Agents·H Giamarellou
Jan 4, 2001·Journal of Clinical Microbiology·B La ScolaD Raoult
Apr 15, 2003·Clinical Microbiology Reviews·Francine Marciano-Cabral, Guy Cabral
Oct 29, 2003·Microbial Pathogenesis·Selwa AlsamNaveed Ahmed Khan
Apr 16, 2004·Clinical Microbiology Reviews·Gilbert Greub, Didier Raoult
May 15, 2004·International Journal of Systematic and Evolutionary Microbiology·Matthew D CollinsEnevold Falsen
Jan 11, 2005·Applied and Environmental Microbiology·Maëlle MolmeretYousef Abu Kwaik
Jun 16, 2006·FEMS Microbiology Reviews·Naveed Ahmed Khan
Dec 13, 2006·International Journal for Parasitology·Suk-Yul JungNaveed Ahmed Khan
Apr 13, 2007·FEMS Immunology and Medical Microbiology·Govinda S VisvesvaraFrederick L Schuster
May 8, 2008·The Journal of Eukaryotic Microbiology·Hadi AbdGunnar Sandström
Oct 14, 2008·Applied and Environmental Microbiology·Michelle A Laskowski-Arce, Kim Orth
Jan 11, 2012·Parasites & Vectors·Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Mar 27, 2012·FEMS Immunology and Medical Microbiology·Lisa OttPaul A Hoskisson
Nov 28, 2012·Parasitology Research·Ruqaiyyah SiddiquiNaveed Ahmed Khan

❮ Previous
Next ❯

Citations

Nov 19, 2017·Applied and Environmental Microbiology·Man Shun Fu, Arturo Casadevall
Jan 24, 2019·Journal of Fungi·Arturo CasadevallPatricia Albuquerque
Aug 26, 2017·Current Microbiology·Scheila S SoaresMarilise B Rott

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.