PMID: 8962075Dec 10, 1996Paper

Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF-RecO-RecR complex in DNA repair and recombination

Proceedings of the National Academy of Sciences of the United States of America
S P HegdeM V Madiraju

Abstract

The products of the recF, recO, and recR genes are thought to interact and assist RecA in the utilization of single-stranded DNA precomplexed with single-stranded DNA binding protein (Ssb) during synapsis. Using immunoprecipitation, size-exclusion chromatography, and Ssb protein affinity chromatography in the absence of any nucleotide cofactors, we have obtained the following results: (i) RecF interacts with RecO, (ii) RecF interacts with RecR in the presence of RecO to form a complex consisting of RecF, RecO, and RecR (RecF-RecO-RecR); (iii) RecF interacts with Ssb protein in the presence of RecO. These data suggested that RecO mediates the interactions of RecF protein with RecR and with Ssb proteins. Incubation of RecF, RecO, RecR, and Ssb proteins resulted in the formation of RecF-RecO-Ssb complexes; i.e., RecR was excluded. Preincubation of RecF, RecO, and RecR proteins prior to addition of Ssb protein resulted in the formation of complexes consisting of RecF, RecO, RecR, and Ssb proteins. These data suggest that one role of RecF is to stabilize the interaction of RecR with RecO in the presence of Ssb protein. Finally, we found that interactions of RecF with RecO are lost in the presence of ATP. We discuss these results to ...Continue Reading

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Citations

Mar 26, 2003·Cell·Susan K Amundsen, Gerald R Smith
Apr 16, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Trudee A TarkowskiFranklin W Stahl
Nov 20, 2008·The Journal of Biological Chemistry·Nodar MakharashviliSergey Korolev
Jul 15, 1997·Nucleic Acids Research·S Ayora, J C Alonso
Apr 16, 2008·Nucleic Acids Research·Michael P Killoran, James L Keck
Nov 14, 2000·Journal of Bacteriology·G A PandyaM Moriya
Mar 29, 2000·Journal of Bacteriology·A R Poteete, A C Fenton
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Justin S LenhartLyle A Simmons
May 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Daiguan YuDonald L Court
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Karen L MaxwellGary J Sharples
Jan 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lyle A SimmonsGraham C Walker
Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Noriko KantakeStephen C Kowalczykowski
Jun 18, 2002·The EMBO Journal·Marie-Agnès Petit, Dusko Ehrlich
Aug 3, 2005·The Journal of Cell Biology·Dawit Kidane, Peter L Graumann
Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Athanasia PavlopoulouAlexandros G Georgakilas
Feb 19, 2005·The EMBO Journal·Ingar LeirosSean McSweeney
Jul 27, 2010·Environmental and Molecular Mutagenesis·John M Hinz
Jan 4, 2013·PLoS Genetics·Paula P CárdenasJuan C Alonso
Oct 22, 2008·Critical Reviews in Biochemistry and Molecular Biology·Robert D SheredaJames L Keck
Oct 11, 2012·Critical Reviews in Biochemistry and Molecular Biology·Dawit KidaneJuan C Alonso
Jul 23, 2019·Nucleic Acids Research·Min Kyung ShinnTimothy M Lohman

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