Abstract
Small unilamellar liposomes composed to dioleoylphosphatidylethanolamine (DOPE) and oleic acid (OA) are stabilized by incubation with normal human serum or plasma [Liu, D., & Huang, L. (1989) Biochemistry 28, 7700-7707]. The present report describes a systematic study of interactions of purified serum proteins and lipoproteins with these liposomes. Albumin destabilized liposomes by extracting OA from the liposomes, whereas immunoglobulins and lipoproteins (HDL, LDL, and VLDL) had no effect. However, HDL and, to some extent, VLDL showed a rapid stabilization activity against the lytic effect of albumin. HDL added together with or shortly after the addition of albumin completely abolished the liposome leakage and aggregation effects induced by albumin. SDS-PAGE analysis of the HDL-stabilized liposomes revealed that apolipoprotein A1 was associated with liposomes. Purified apolipoprotein A1, but not a lipid mixture resembling the lipid composition of HDL, showed comparable liposome stabilization activity as HDL. Furthermore, synthetic peptides resembling the amphipathic helices found in apolipoprotein A1 also showed strong liposome stabilization activity. Peptides which were able to form amphipathic helixes of a wedge shape were m...Continue Reading
Citations
Nov 1, 1995·Journal of Magnetic Resonance Imaging : JMRI·R W StorrsK C Li
Apr 1, 1992·The International Journal of Biochemistry·T Cserháti, M Szögyi
Aug 14, 1992·Biochimica Et Biophysica Acta·D C Litzinger, L Huang
Dec 21, 1993·Biochimica Et Biophysica Acta·J G SmithJ B German
Jul 26, 1991·Biochimica Et Biophysica Acta·P Hug, R G Sleight
Nov 18, 2000·Progress in Lipid Research·D C DrummondJ Leroux
Jun 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·J LerouxD C Drummond
Feb 17, 2000·International Journal of Pharmaceutics·R Galántai, I Bárdos-Nagy
Jul 25, 2003·Magnetic Resonance Imaging·Knut-Egil LøklingJo Klaveness
Aug 5, 1999·The Biochemical Journal·M MotizukiK Tsurugi
Sep 27, 2008·Expert Opinion on Drug Delivery·Malavosklish Bikram, Jennifer L West
Nov 11, 2009·Journal of Pharmaceutical Sciences·Brian BannoEllen K Wasan
Apr 12, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kenji KonoToru Takagishi
Nov 8, 2013·The Protein Journal·Gloria CeledónEduardo Lissi
Jul 13, 1994·Biochimica Et Biophysica Acta·A AriënB Dupuy
Apr 8, 1993·Biochimica Et Biophysica Acta·P VianiB Cestaro
Apr 1, 1992·Analytical Biochemistry·D Liu, L Huang
Nov 18, 2005·International Journal of Pharmaceutics·Clement MugabeAbdelwahab Omri
Jan 28, 2014·Colloids and Surfaces. B, Biointerfaces·Haiqing YinYou Han Bae
Sep 22, 2018·Journal of Drug Targeting·Kamel S AhmedChen Jinghua
Oct 1, 2005·Journal of Liposome Research·Xuedong YanJan A A M Kamps
Jan 27, 1995·The Journal of Biological Chemistry·V K MishraG M Anantharamaiah
May 1, 1992·International Journal of Peptide and Protein Research·S KimuraR Schwyzer
Jul 10, 2008·Molecular Pharmaceutics·Shyh-Dar Li, Leaf Huang
Aug 11, 2018·Biomaterials·Hoon ChoiI-Wei Chen