Interactions of SR45, an SR-like protein, with spliceosomal proteins and an intronic sequence: insights into regulated splicing

The Plant Journal : for Cell and Molecular Biology
Irene S DayAnireddy S N Reddy

Abstract

SR45 is a serine/arginine-rich (SR)-like protein with two arginine/serine-rich (RS) domains. We have previously shown that SR45 regulates alternative splicing (AS) by differential selection of 5' and 3' splice sites. However, it is unknown how SR45 regulates AS. To gain mechanistic insights into the roles of SR45 in splicing, we screened a yeast two-hybrid library with SR45. This screening resulted in the isolation of two spliceosomal proteins, U1-70K and U2AF(35) b that are known to function in 5' and 3' splice site selection, respectively. This screen not only confirmed our prior observation that U1-70K and SR45 interact, but also helped to identify an additional interacting partner (U2AF(35) ). In vitro and in vivo analyses revealed an interaction of SR45 with both paralogs of U2AF(35) . Furthermore, we show that the RS1 and RS2 domains of SR45, and not the RNA recognition motif (RRM) domain, associate independently with both U2AF(35) proteins. Interaction studies among U2AF(35) paralogs and between U2AF(35) and U1-70K revealed that U2AF(35) can form homo- or heterodimers and that U2AF(35) proteins can associate with U1-70K. Using RNA probes from SR30 intron 10, whose splicing is altered in the sr45 mutant, we show that SR45...Continue Reading

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Citations

Jan 9, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Braden WaltersXiang Jia Min
Nov 2, 2013·The Plant Cell·Anireddy S N ReddyAndrea Barta
Oct 19, 2013·Nucleic Acids Research·Katarzyna Dorota RaczynskaArtur Jarmolowski
Jun 12, 2014·International Journal of Molecular Sciences·Shengming YangHongyan Zhu
Aug 24, 2012·The Plant Journal : for Cell and Molecular Biology·Julie ThomasAnireddy S N Reddy
Apr 4, 2015·Current Opinion in Plant Biology·Sergei FilichkinTodd C Mockler
Oct 30, 2016·BMC Genomics·Delasa AghamirzaieRuth Grene
Sep 25, 2016·The Plant Journal : for Cell and Molecular Biology·Yu LingMagdy M Mahfouz
Feb 24, 2017·International Journal of Molecular Sciences·Xudong ShangLigeng Ma
Dec 14, 2017·Nature Communications·Jie HuangSuomeng Dong
Sep 2, 2020·Biochemical Society Transactions·Paola PunzoGiorgia Batelli
Apr 11, 2019·Plant Molecular Biology·Mohammed AlbaqamiAnireddy S N Reddy
Feb 23, 2019·Nucleic Acids Research·Ibtissam JabreNaeem H Syed
Oct 22, 2019·Frontiers in Plant Science·Ying Cao, Ligeng Ma

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