Interactions of the acyl chain with the Saccharomyces cerevisiae acyl carrier protein

Biochemistry
Daniel R PerezGerhard Wider

Abstract

Acyl carrier protein (ACP) domains are critical integral components of multifunctional type I fatty acid synthases (FAS I) and polyketide synthases (PKSs), where they shuttle the growing adducts of the synthesis between the catalytic domains. In contrast to ACP of mammalian FAS I, PKSs, and the dissociated fatty acid synthase type II systems (FAS II) of bacteria, fungal FAS I ACP consists of two subdomains, one comprising the canonical ACP fold observed in all FAS systems and the other representing an extra structural subdomain. While ACPs of dissociated FAS II are able to sequester the reaction intermediates during substrate shuttling, such a transport mechanism has not been observed in ACP domains of multifunctional FAS I and PKS systems. For a better understanding of the interaction between the canonical subdomain of fungal ACP with the growing acyl chain and the role of the structural subdomain, we determined the structure of the isolated Saccharomyces cerevisiae acyl carrier protein (ScACP) domain by NMR spectroscopy and investigated the interactions between ScACP and covalently attached substrate acyl chains of varying length by monitoring chemical shift perturbations. The interactions were mapped to the hydrophobic core ...Continue Reading

References

Dec 1, 1980·European Journal of Biochemistry·F Lynen
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P W MAJERUSP R VAGELOS
Jun 15, 2005·Annual Review of Biochemistry·Stephen W White Rock
Oct 4, 2005·Journal of Computational Chemistry·David A CaseRobert J Woods
Feb 24, 2006·Journal of the American Chemical Society·Gerhard Wider, Lars Dreier
Mar 4, 2006·Science·Simon JenniNenad Ban
Apr 19, 2006·Biochemistry·Gregory A ZornetzerJohn L Markley
Oct 24, 2007·Journal of Biomolecular NMR·Frank H SchumannHans Robert Kalbitzer
Nov 1, 2007·The Journal of Biological Chemistry·Eliza PloskońMatthew P Crump
Dec 7, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David M Byers, Huansheng Gong
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrik JohanssonMartin Grininger
Sep 6, 2008·Science·Timm MaierNenad Ban
Oct 25, 2008·Current Opinion in Structural Biology·Marc LeibundgutNenad Ban
Feb 9, 2010·Biochemistry·Pakorn Wattana-amornMatthew P Crump
Aug 25, 2010·Quarterly Reviews of Biophysics·Timm MaierNenad Ban
Jan 8, 2013·Journal of Molecular Biology·Daniel BoehringerMarc Leibundgut

❮ Previous
Next ❯

Citations

Aug 24, 2018·Natural Product Reports·Dominik A HerbstTimm Maier
Jul 12, 2017·Beilstein Journal of Organic Chemistry·Manuel Fischer, Martin Grininger
Jul 11, 2019·PloS One·Rohit FarmerPeter James Winn
Mar 26, 2019·Chembiochem : a European Journal of Chemical Biology·Christina S HeilMartin Grininger
May 31, 2020·Communications Biology·Jennifer W Lou, Mohammad T Mazhab-Jafari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.