Interactions of the borna disease virus P, N, and X proteins and their functional implications.

The Journal of Biological Chemistry
M SchwemmleW I Lipkin

Abstract

Borna disease virus (BDV) causes persistent central nervous system infection and behavioral disturbances in warm-blooded animals. Protein interaction studies were pursued to gain insight into the functions of the putative nucleoprotein (N), phosphoprotein (P), atypical glycoprotein (gp18), and X protein (X) of BDV. Coimmunoprecipitation experiments indicated that N and P, and P and X, form complexes in infected cells. Two-hybrid analyses confirmed interactions between P and P, P and X, and P and N, but not between P and gp18, N and gp18, X and gp18, or X and N. Analysis of P truncation mutants identified three nonoverlapping regions important for oligomerization (amino acids (aa) 135-172), and binding to X (aa 33-115) or N (aa 197-201). Coexpression of X stimulated oligomerization of P but decreased N-P complex formation. Immunocytochemistry of transfected noninfected CHO cells demonstrated that the distribution of X is dependent upon the presence of P-X expressed alone was found predominantly in the cytoplasm whereas coexpression of X and P resulted in nuclear localization. Immunocytochemistry of infected cells revealed nuclear colocalization of P and X. Interactions of P, N, and X may have implications for regulation of BDV t...Continue Reading

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Citations

May 12, 2005·Virus Research·Daniel Gonzalez-DuniaMartin Schwemmle
May 24, 2000·Journal of Virology·N NowotnyM Schwemmle
Feb 7, 2001·Journal of Virology·M P WalkerW I Lipkin
Mar 6, 2010·Emerging Infectious Diseases·Patricia GrayIan Tizard
Aug 24, 2013·Viruses·Tomoyuki Honda, Keizo Tomonaga
Aug 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kan FujinoKeizo Tomonaga
Jun 1, 2006·The Veterinary Quarterly·S Kamhieh, R L P Flower
Aug 14, 2013·Biochemical and Biophysical Research Communications·Wuqi SongFengmin Zhang
Dec 1, 2009·Virology·Miriam HockWinfried Weissenhorn
Oct 7, 2003·Structure·Markus G RudolphRalf Ficner
May 24, 2016·Animal Health Research Reviews·Ian TizardSusan Payne
Aug 20, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Vanessa ZimmermannDennis Rubbenstroth
Jun 26, 2004·The Journal of General Virology·Marion PoenischUrs Schneider
Oct 18, 2005·The Journal of General Virology·Malte SchwardtMartin Schwemmle
Mar 10, 2001·Reviews in Medical Virology·I Jordan, W I Lipkin

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