Interactions of the human calcitonin fragment 9-32 with phospholipids: a monolayer study

Biophysical Journal
Kerstin WagnerF Heitz

Abstract

Human calcitonin and its C-terminal fragment 9-32 (hCT(9-32)) administered in a spray translocate into respiratory nasal epithelium with an effect similar to intravenous injection. hCT(9-32) is an efficient carrier to transfer the green fluorescent protein into excised bovine nasal mucosa. To understand the translocation of hCT(9-32) across plasma membranes, we investigated its interactions with phospholipids and its interfacial structure using model lipid monolayers. A combination of physicochemical methods was applied including surface tension measurements on adsorbed and spread monolayers at the air-water interface, Fourier transform infrared, circular dichroism, and atomic force microscopy on Langmuir-Blodgett monolayers. The results disclose that hCT(9-32) preferentially interacts with negatively charged phospholipids and does not insert spontaneously into lipid monolayers. This supports a nonreceptor-mediated endocytic internalization pathway as previously suggested. Structural studies revealed a random coil conformation of hCT(9-32) in solution, transforming to alpha-helices when the peptide is localized at lipid-free or lipid-containing air-water interfaces. Atomic force microscopy studies of monolayers of the peptide a...Continue Reading

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Citations

Mar 30, 2007·European Biophysics Journal : EBJ·Daniela MeleleoSilvia Micelli
Dec 28, 2007·Advanced Drug Delivery Reviews·Robert RennertAnnette G Beck-Sickinger
May 12, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·J Fernández-CarneadoE Giralt
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Jan 22, 2008·ChemMedChem·Robert RennertAnnette G Beck-Sickinger
Aug 23, 2020·Nitric Oxide : Biology and Chemistry·Huixian YeZhonghong Gao
Nov 9, 2010·The Journal of Physical Chemistry. B·Tania Sheynis, Raz Jelinek
Mar 8, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Christian BijaniErick J Dufourc
Sep 22, 2018·Bioconjugate Chemistry·Kate J F CarnevaleGeoffrey F Strouse

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