Interactions with single-stranded and double-stranded DNA-binding factors and alternative promoter conformation upon transcriptional activation of the Htf9-a/RanBP1 and Htf9-c genes.

The Journal of Biological Chemistry
G Di MatteoP Lavia

Abstract

The murine Htf9-a/RanBP1 and Htf9-c genes are divergently transcribed from a shared TATA-less promoter. Transcription of both genes is initiated on complementary DNA strands and is controlled by cell cycle-dependent mechanisms. The bidirectional promoter harbors a genomic footprint flanking the major transcription start site of both genes. Transient promoter assays showed that the footprinted element is important for transcription of both genes. Protein-binding experiments and antibody assays indicated that members of the retinoid X receptor family interact with the double-stranded site. In addition, distinct factors interact with single DNA strands of the element. Double-stranded binding factors were highly expressed in liver cells, in which neither gene is transcribed, while single-stranded binding proteins were abundant in cycling cells, in which transcription of both genes is efficient. In vivo S1 analysis of the promoter depicted an S1-sensitive organization in cells in which transcription of both genes is active; S1 sensitivity was not detected in conditions of transcriptional repression. Thus, the same element is a target for either retinoid X receptor factors, or for single-stranded binding proteins, and form distinct c...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S R WileyJ E Mertz
Nov 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L Weis, D Reinberg
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L A LaiminsP Gruss
Apr 7, 1995·The Journal of Biological Chemistry·L BazarM Avigan
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S L AckermanC Y Yeung
Jul 1, 1993·Genes & Development·N Hernandez
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Dec 15, 1995·Cell·D J Mangelsdorf, R M Evans
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Tomonaga, D Levens
Feb 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M G RushP D'Eustachio
Oct 1, 1996·Current Opinion in Genetics & Development·S Minucci, K Ozato
Nov 1, 1996·Genes & Development·G OrphanidesD Reinberg
Aug 8, 1997·Cell·T Perlmann, R M Evans
Mar 1, 1996·Trends in Cell Biology·S Sazer

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