Interactions within the yeast Sm core complex: from proteins to amino acids.

Molecular and Cellular Biology
A CamassesR Bordonné

Abstract

Sm core proteins play an essential role in the formation of small nuclear ribonucleoprotein particles (snRNPs) by binding to small nuclear RNAs and participating in a network of protein interactions. The two-hybrid system was used to identify SmE interacting proteins and to test for interactions between all pairwise combinations of yeast Sm proteins. We observed interactions between SmB and SmD3, SmE and SmF, and SmE and SmG. For these interactions, a direct biochemical assay confirmed the validity of the results obtained in vivo. To map the protein-protein interaction surface of Sm proteins, we generated a library of SmE mutants and investigated their ability to interact with SmF and/or SmG proteins in the two-hybrid system. Several classes of mutants were observed: some mutants are unable to interact with either SmF or SmG proteins, some interact with SmG but not with SmF, while others interact moderately with SmF but not with SmG. Our mutational analysis of yeast SmE protein shows that conserved hydrophobic residues are essential for interactions with SmF and SmG as well as for viability. Surprisingly, we observed that other evolutionarily conserved positions are tolerant to mutations, with substitutions affecting binding to...Continue Reading

References

Aug 1, 1992·Seminars in Cell Biology·E Izaurralde, I W Mattaj
Sep 25, 1992·Nucleic Acids Research·L Good, R N Nazar
Oct 25, 1991·Nucleic Acids Research·J HillG Donald
Nov 30, 1990·Biochimica Et Biophysica Acta·R LührmannM Bach
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·L Breeden, K Nasmyth
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·T LehmeierR Lührmann
Aug 30, 1994·Biochemistry·J R Patton
Dec 24, 1993·Science·S Kandels-Lewis, B Séraphin
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·G NeubauerM Mann

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Citations

Feb 19, 2010·Translational Oncology·Isabela Werneck CunhaLuiz Fernando Lima Reis
Aug 11, 2004·Experimental Cell Research·Cyrille GirardRémy Bordonné
Nov 3, 1998·The EMBO Journal·F Caspary, B Séraphin
Jul 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z PalfiA Bindereif
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·T AchselR Lührmann
Apr 17, 2008·Molecular Systems Biology·Arun K RamaniEdward M Marcotte
Sep 16, 2006·The EMBO Journal·Pingping WangAlbrecht Bindereif
Oct 12, 2000·Molecular and Cellular Biology·R Bordonné
Feb 23, 2000·Journal of Virology·T M Soliman, S J Silverstein
Jun 10, 2021·Journal of Experimental Botany·Varvara DikayaMarkus Schmid

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